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Safety Data Sheet (SDS) PE Anti-rat CD4 Antibody     Product Data Sheet (PDF)    
PE Anti-rat CD4 Antibody
1607535 50 µg $110.00       
Clone: W3/25
Isotype: Mouse IgG1, κ
Reactivity: Rat
Immunogen: Rat thymocyte membrane glycoproteins
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.5% BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

The W3/25 antibody has been shown to inhibit IL-2 production by T helper cells and to prevent autoimmune T cell transfer in an MBP induced EAE model in vivo. Additional reported applications (for the relevant formats) include: immunohistochemistryof acetone-fixed frozen sections1,2, inhibition of IL-2 production3, inhibition of MBP-induced T cell activation in EAE transfer model3.

Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References:

1. Whiteland JL, et al. 1995. J. Histochem. Cytochem. 43:313. (IHC)
2. Shioji K, et al. 2001. Circulation Res. 89:540. (IHC)
3. Mannie MD, et al. 1993. J. Immunol. 151:7293.
4. Kurtz CC, et al. 2007. Dev. Comp. Immunol. 31:415. PubMed

LOU rat splenocytes stained with

LOU rat splenocytes stained with W3/25 PE



Description:

CD4 is a 55 kD glycoprotein also known as T4. Rat CD4 is a member of the immunoglobulin superfamily and is expressed on majority of thymocytes, macrophages, and a peripheral T cell subset (T helper cells). CD4 is a T cell co-receptor that interacts with the MHC class II molecule and is involved in T cell activation. 

Other Names: T4, L3T4, W3/25
Structure: Ig superfamily, 55 kD
Distribution: Majority of thymocytes, macrophages, and a peripheral T cell subset (T helper cells)
Function: T cell co-receptor, T cell activation
Ligand Receptor: MHC class II molecule
Antigen References:

1. Brideau RJ, et al. 1980. Eur. J. Immunol. 10:609.
2. Clark SJ, et al. 187. P. Natl. Acad. Sci. USA 84:1649.