Sony Biotechnology Inc. Sony Biotechnology Inc.cart
About Us Products What's New Library News Calendar Contact Us Place Order
Safety Data Sheet (SDS) FITC Anti-rat CD8a Antibody     Product Data Sheet (PDF)    
FITC Anti-rat CD8a Antibody
1608515 100 µg $125.00       
Clone: OX-8
Isotype: Mouse IgG1, κ
Immunogen: High molecular weight glycoproteins from rat thymocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.5% BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

The OX-8 antibody has been reported to partially block T cell responses, including mixed lymphocyte reactions and cytotoxic T cell responses, and to induce macrophage activation. Additional reported applications (for the relevant formats) include: immunohistochemistry1,2 of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections, immunoprecipitation3, in vivo and in vitro blocking of T cell responses3, 4, macrophage stimulation5, and Western blotting3,6.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Barclay AN. 1981. Immunology 42:593. (IHC)
2. Wallgren AC, et al. 1995. Transplantation 60:594. (IHC)
3. Torres-Nagel N, et al. 1992. Eur. J. Immunol. 22:2841. (IP, WB)
4. Mason DW, et al. 1983. Immunol. Rev. 74:57.
5. Hirji N, et al. 1997. J. Immunol. 158:1833. (FA)
6. Mitnacht R,, et al. 1998. J. Immunol. 160:700. (WB)

LOU rat splenocytes stained with

LOU rat splenocytes stained with OX-8 FITC



Description:

CD8a is a 32 kD glycoprotein also known as T8, Lyt2, Ly-2, and CD8α. CD8a is a member of the immunoglobulin superfamily expressed on most thymocytes, subset of mature T cells, most NK cells, macrophages, and some activated CD4+ T cells (not resting). CD8a forms heterodimers with the CD8β chain (CD8b) on the surface of most thymocytes, while mature peripheral T lymphocytes express almost exclusively the CD8 αβ heterodimer. Intestinal intraepithelial lymphocytes express CD8a without CD8b. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 participates in T cell activation through its association with the T cell receptor complex and protein tyrosine kinase lck (p56lck).

Other Names: CD8a, T8, Leu-8, Ly-2, Lyt2
Structure: Ig superfamily, approximately 32 kD
Distribution: Thymocytes, NK cells, most CD8+ cells, intestinal intraepithelial lymphocytes, activated CD4+ cells, macrophages
Function: T cell activation, co-receptor for T cell activation
Ligand Receptor: MHC class I molecule
Antigen References:

1. Johnson P, et al. 1985. EMBO J. 4:2539.
2. Thomas ML, et al. 1983. Eur. J. Immunol. 13:855.