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Safety Data Sheet (SDS) Purified Anti-rat CD11a Antibody     Product Data Sheet (PDF)    
Purified Anti-rat CD11a Antibody
1609505 100 µg $100.00       
Clone: WT.1
Isotype: Mouse IgG2a, κ
Reactivity: Rat
Immunogen: PHA-stimulated rat splenocytes and rat thymic lymphoma FTL-43
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application:

FC - Quality tested
IHC, IP - Reported in the literature

Application Notes:

The WT.1 antibody recognizes both activated and unactivated LFA-1 and inhibits the binding of LFA-1 to ICAM-1. The WT.1 antibody has been shown to inhibit the mixed lymphocyte reaction, leukocyte infiltration, and graft rejection. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections1,2, immunoprecipitation3, in vivo and in vitro blocking of cellular adhesion3-6.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Andrews FJ, et al. 1997. Dig. Dis. Sci. 42:326. (IHC)
2. Springer TA. 1994. Cell 76:301. (IHC)
3. Tamatani T, et al. 1991. Eur. J. Immunol. 21:627. (IP Block)
4. Devine L, et al. 1996. Immunol. 88:456. (Block)
5. Ohta Y, et al. 1998. Transplantation 65:1094. (Block)
6. Watanabe T, et al. 1997. Am. J. Pathol. 150:971. (Block)

LOU rat splenocytes stained with

LOU rat splenocytes stained with purified WT.1, followed by anti-mouse IgG2a FITC



Description:

CD11a is a 160-170 kD glycoprotein also known as αL integrin, or LFA-1 α chain. It is a member of the integrin family and forms a heterodimer with β2 integrin (CD18). The LFA-1 (CD11a/CD18) is broadly expressed on leukocytes and mediates intercellular adhesion and cellular activation. CD54 (ICAM-1), CD102 (ICAM-2), and CD50 (ICAM-3) are the ligands of LFA-1.

Other Names: LFA-1 α chain, αL Integrin, ITGAL
Structure: Integrin family member, 160-170 kD, associates with integrin β2
Distribution: Lymphocytes, granulocytes, monocytes, macrophages
Function: Cellular adhesion
Ligand Receptor: ICAM-1, ICAM-2, ICAM-3
Antigen References:

1. Tamatani T, et al. 1991. Eur. J. Immunol. 21:627.
2. Tamatani T, et al. 1991. Eur. J. Immunol. 21:855.