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Safety Data Sheet (SDS) PE Anti-rat CD25 Antibody     Product Data Sheet (PDF)    
PE Anti-rat CD25 Antibody
1610525 50 µg $110.00       
Clone: OX-39
Isotype: Mouse IgG1, κ
Reactivity: Rat
Immunogen: Rat T cell blasts from mixed lymphocyte reactions
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.5% BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: immunohistochemistryof acetone-fixed frozen sections1, immunoprecipitation1, weakly blocks IL-2 binding1, 2, 3, and blocks IL-2 induced epithelial cell migration3.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Peterson DJ, et al. 1987. Mol. Immunol. 24:1281. (IHC, IP, Block)
2. Tellides G, et al. 1987. Transplant Proc. 19:4231. (Block)
3. Digness AU, et al. 1996. Exp.Cell Res. 225:422. (Block)

ConA-stimulated (3day) LOU splenocytes stained

ConA-stimulated (3day) LOU splenocytes stained with OX-39 PE



Description:

CD25 is a 55 kD glycoprotein also known as IL-2 receptor α chain. It is broadly expressed on activated T and B cells, a subset of thymic and splenic dendritic cells, and intestinal epithelial cells in the rat. IL-2 is a critical cytokine involved in lymphocyte proliferation and clonal expansion. IL-2 signaling requires the high affinity IL-2 receptor composed of IL-2 receptor chains α, β, and γ. The OX-39 antibody weakly inhibits IL-2 binding to the IL-2 receptor and has been shown to block some IL-2 mediated responses in vitro.

Other Names: IL-2 receptor chain α, IL-2Rα, ?low affinity IL-2 receptor chain, Tac, p55, Ly-43
Structure: Type I membrane glycoprotein, 55 kD
Distribution: Activated T cells and B cells, thymic and splenic dendritic cells, intestinal epithelial cells
Function: Low affinity receptor for IL-2; associates with IL-2 receptor β (CD122) and γ (CD132) chains to form high affinity receptor
Ligand Receptor: IL-2
Antigen References:

1. Digness AU, et al. 1996. Exp.Cell Res. 225:422.
2. Peterson DJ, et al. 1987. Mol. Immunol. 24:1281.