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Safety Data Sheet (SDS) PE Anti-mouse CD70 Antibody     Product Data Sheet (PDF)    
PE Anti-mouse CD70 Antibody
1123025 50 µg $100.00       
1123030 200 µg $255.00       
Clone: FR70
Isotype: Rat IgG2b, κ
Reactivity: Mouse
Immunogen: BALB/c mouse B lymphoma A20.2J
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro blocking of CD70-CD27 binding and inhibition of T and NK cell responses1-3 , and immunohistochemistry4 of aceton-fixed frozen sections. For the most successful immunofluorescent staining results, it may be necessary to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody or biotinylated anti-rat IgG second step, followed by SAv-PE ). To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 , is recommended prior to immunofluorescent staining.

Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References:

1. Oshima H, et al. 1998. Int. Immunol. 10:517. (Block IP)
2. Akiba HH, et al. 1999. J. Immunol. 162:7058. (Block)
3. Takeda K, et al. 2000. J. Immunol. 164:1741. (Block)
4. Henke A, et al. 2000. J. Virol. 74:4284. (IHC)
5. Brown SL, et al. 2007. J. Clin. Invest. 117:258.

Mouse B cell line A20

Mouse B cell line A20 stained with FR70 PE



Description:

CD70 is a type II transmembrane protein. It is a member of the TNF superfamily, expressed on activated B cells, activated dendritic cells, and some activated T cells (low levels). CD70 interacts with CD27 to promote T-B cell cross-stimulation and co-stimulate B cell proliferation and immunoglobulin production. Cells expressing CD70 can co-stimulate T cell proliferation and enhance the production of cytokines. The FR70 antibody blocks CD70 binding to CD27.

Other Names: Ki-24, CD27-ligand, TNFSF7
Structure: TNF/NGF superfamily, 30-33 kD
Distribution: Activated B cells and some activated T cells
Function: T-B cell cross-stimulation
Ligand Receptor: CD27
Antigen References:

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Tesselaar K, et al. 1997. J. Immunol. 159:4959.
3. Akiba H, et al. 1999. J. Immunol. 162:7058.