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Safety Data Sheet (SDS) Biotin Anti-rat CD54 Antibody     Product Data Sheet (PDF)    
Biotin Anti-rat CD54 Antibody
1612015 100 µg $125.00       
Clone: 1A29
Isotype: Mouse IgG1, κ
Reactivity: Rat
Immunogen: HEV-derived cell line Ax
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application:

FC - Quality tested
IHC - Reported in the literature

Application Notes:

Additional reported applications (for the relevant formats) include: immunohistochemistry1,5 of acetone-fixed frozen sections and STUF-treated paraffin-embedded sections, immunoprecipitation2, in vitro and in vivo blocking of PMA-induced T cell blast aggregation and adhesion to HEV-derived endothelial cells and purified ICAM-1 protein2,3,4.

Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References:

1. Clark WM, et al. 1995. Mol. Chem. Neuropathol. 26:213. (IHC)
2. Tamatani T, et al. 1990. Int. Immunol. 2:165. (IP)
3. Tamatani T, et al. 1991. Eur. J. Immunol. 21:627.
4. Willenborg DO, et al. 1996. J. Immunol. 157:1973.
5. McKechnie NM, et al. 2002. IVOS 43:411. (IHC)

LOU rat splenocytes stained with

LOU rat splenocytes stained with biotinylated 1A29, followed by SAV-PE



Description:

CD54 is an 85 kD protein also known as ICAM-1. It is a member of the immunoglobulin superfamily that is expressed on high endothelial venules in lymphoid tissue, peripheral blood monocytes, dendritic cells, granulocytes and thymic stromal cells. CD54 is weakly expressed on T and B cells, although expression can be increased by activation (especially on endothelial cells). ICAM-1 is an important protein in cell adhesion, inflammation and immune responses. CD54 has been shown to bind to CD11a/CD18 (LFA-1), CD11b/CD18, CD11c/CD18, CD43, fibrinogen, and hyaluronan. The 1A29 antibody has been reported to block T cell aggregation and binding of lymphocytes to HEV-derived endothelial cells and purified ICAM-1 protein.

Other Names: ICAM-1
Structure: Ig superfamily, 85 kD
Distribution: High endothelial venules in lymphoid tissues, peripheral blood monocytes, dendritic cells, granulocytes, thymic stromal cells and weakly on T and B cells. Upregulated on activated cells and endothelial cells.
Function: Cellular adhesion, inflammation, immune reactions
Ligand Receptor: CD11a/CD18 (LFA-1), CD11b/CD18, CD11c/CD18, CD43, fibrinogen, hyaluronan
Antigen References:

1. Tamatani T, et al. 1990. Int. Immunol. 2:165.
2. Tamatani T, et al. 1991. Eur. J. Immunol. 21:627.
3. Turunen JP, et al. 1993. Scand. J. Immunol. 37:282.