The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Storage & Handling:
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Additional reported applications (for the relevant formats) include immunoprecipitation2.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
1. Ito K, et al. 2000. Proc. Natl. Sci. USA 97:740. 2. Okada CY, et al. 1990. J. Immunol. 144:3473.
Balb/c mouse splenocytes stained with CD3 (145-2C11) APC and TR310 FITC
The TR310 antibody reacts with the mouse T cell receptor Vβ7 segment . T cells expressing the α/β T cell receptor use clonally distributed TCRs to recognize cell-bound antigens such as peptide/MHC complexes and viral superantigens. The α/β TCR is an oligomeric TCR complex composed of covalently bound α and β chains involved in antigen recognition and the non-covalently associated monomorphic proteins CD3δ, γ, ε, and ζ chains. The TR310 antibody reacts with one such clonally distributed TCR β chain known as Vβ7. Vβ7 is expressed on a small subpopulation of both CD4+ and CD8+ T cells. The TR310 antibody has been used for the identification of Vβ7 expressing T cells by flow cytometry and immunoprecipitation.
T cell receptor Vβ7 chain
Ig superfamily member, TCR α/β with CD3 forms the CD3/TCR complex
Expressed on a subpopulation of CD4+ and CD8+ T cells bearing the Vβ7 T cell receptor segment
Antigen recognition, T cell activation
Peptide bound to MHC
Peptide bound to MHC
1. Okada CY, et al. 1990. J. Immunol. 144:3473.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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