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Safety Data Sheet (SDS) PE Anti-mouse TCR Vbeta7 Antibody     Product Data Sheet (PDF)    
PE Anti-mouse TCR Vβ7 Antibody
1191535 25 µg $90.00       
1191540 100 µg $175.00       
Clone: TR310
Isotype: Rat IgG2b, κ
Reactivity: Mouse TCR Vβ7 bearing T cells and NKT cells; may not be present in mice expressing the MIS-1a haplotype
Immunogen: T cell lymphoma TK-1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Additional reported applications (for the relevant formats) include immunoprecipitation2.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Ito K, et al. 2000. Proc. Natl. Sci. USA 97:740.
2. Okada CY, et al. 1990. J. Immunol. 144:3473.
3. Huang JR, et al. 2014. J Immunol. 192:1972. PubMed

BALB/c splenocytes stained with CD3

BALB/c splenocytes stained with CD3 (145-2C11) APC and TR310 PE



Description:

The TR310 antibody reacts with the mouse T cell receptor Vβ7 segment . T cells expressing the α/β T cell receptor use clonally distributed TCRs to recognize cell-bound antigens such as peptide/MHC complexes and viral superantigens. The α/β TCR is an oligomeric TCR complex composed of covalently bound α and β chains involved in antigen recognition and the non-covalently associated monomorphic proteins CD3δ, γ, ε, and ζ chains. The TR310 antibody reacts with one such clonally distributed TCR β chain known as Vβ7. Vβ7 is expressed on a small subpopulation of both CD4+ and CD8+ T cells. The TR310 antibody has been used for the identification of Vβ7 expressing T cells by flow cytometry and immunoprecipitation.

Other Names: T cell receptor Vβ7 chain
Structure: Ig superfamily member, TCR α/β with CD3 forms the CD3/TCR complex
Distribution: Expressed on a subpopulation of CD4+ and CD8+ T cells bearing the Vβ7 T cell receptor segment
Function: Antigen recognition, T cell activation
Ligand Receptor: Peptide bound to MHC
Receptors: Peptide bound to MHC
Antigen References:

1. Okada CY, et al. 1990. J. Immunol. 144:3473.