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Safety Data Sheet (SDS) Purified Anti-mouse CD90 Antibody     Product Data Sheet (PDF)    
Purified Anti-mouse CD90 Antibody
1126005 50 µg $50.00       
1126010 500 µg $155.00       
Clone: G7
Isotype: Rat IgG2c, κ
Reactivity: Mouse
Immunogen: Mouse T-cell hybridoma C6/G8
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application:

FC - Quality tested
IHC - Reported in the literature

Application Notes:

Clone G7 binds to Thy-1 epitope region A and also recognizes Thy-1.1/CD90.1 and Thy-1.2/CD90.2 alloantigens. Additional reported applications (for the relevant formats) include: in vitro T-cell activation1,3-4,6, induction of apoptosis2,5, and immunohistochemical staining7,8 of zinc-fixed paraffin-embedded sections and acetone-fixed frozen sections.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Gunter KC, et al. 1984. J. Exp. Med. 159:716. (Activ)
2. Hueber AO, et al. 1994. J. Exp. Med. 179:785. (Apop)
3. Tentori L, et al. 1988. J. Immunol. 140:1089. (Activ)
4. Sugiyama E, et al. 1990. Cell. Immunol. 130:271. (Activ)
5. Ucker DS, et al. 1992. J. Immunol. 149:1583. (Apop)
6. Kruisbeek AM, et al. 1991. In Current Protocols in Immunology. pp. 3.12.1. (Activ)
7. Nitta H, et al. 1997. Cell Vision 4:73. (IHC)
8. Whiteland JL, et al. 1995. J. Histochem. Cytochem. 43:313. (IHC)

C57BL/6 splenocytes stained with purified

C57BL/6 splenocytes stained with purified G7, followed by anti-rat IgG FITC



Description:

CD90 is a 25-35 kD immunoglobulin superfamily member, also known as Thy-1. It is expressed on hematopoietic stem cells and neurons, all thymocytes, and peripheral T cells, but not on B cells. CD90 is a glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein involved in signal transduction. CD90 is involved in costimulation of lymphocyte proliferation and activation, and hematopoietic stem cell differentiation. CD90 has been shown to interact with CD45. The G7 antibody is able to stimulate T cell proliferation and IL-2 secretion and induce apoptosis of thymocytes and CTL clones.

Other Names: Thy-1, Thy1
Structure: Ig superfamily, 25-35 kD
Distribution: Hematopoietic stem cells and neurons, all thymocytes, peripheral T cells
Function: Lymphocyte costimulation, proliferation and differentiation of hematopoietic stem cells
Ligand Receptor: CD45
Antigen References:

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Craig W, et al.1993 J. Exp. Med. 177:1331.
3. Mayani H, et al. 1994 Blood 83:2410.