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Safety Data Sheet (SDS) Biotin Anti-mouse CD273 B7-DC PD-L2 Antibody     Product Data Sheet (PDF)    
Biotin Anti-mouse CD273 (B7-DC, PD-L2) Antibody
1136015 50 µg $100.00       
Clone: TY25
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: Mouse B7-DC transfected cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: immunoprecipitation1, Western blotting1,6, blocking2,4,5 of PD-1 mediated interactions, and immunohistochemistry of acetone-fixed frozen sections3.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Yamazaki T, et al. 2002. J. Immunol. 169:5538. (FC, IP, WB)
2. Ansari MJI, et al. 2003. J. Exp. Med. 198:63. (Block)
3. Salama AD, et al. 2003. J. Exp. Med. 198:71. (IHC)
4. Matsumoto K, et al. 2004. J. Immunol. 172:2530. (FC, Block)
5. Yamazaki T, et al. 2005. J. Immunol. 175:1586. (Block)
6. Meng Q, et al. 2006. Invest Opthalmol Vis Sci. 47:444. (WB) PubMed
7. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed

mB7-DC transfected cells stained with

mB7-DC transfected cells stained with biotinylated TY25, followed by Sav-PE



Description:

B7-DC is also called programmed death ligand 2 (PDL2). It has recently been clustered as CD273. This ligand is a 42 kD member of the immunoglobulin receptor superfamily expressed on a subset of dendritic cells, liver and a small subset of macrophages as well as a few transformed cell lines. CD273 has been reported to be stimulatory on dendritic cells when cross-linked and to inhibit T cell activation upon engaging the PD-1 receptor. CD273 has also been reported to bind to an alternative receptor and to mediate T cell activation through such non-PD1 mediated interactions. The TY25 antibody has been reported to be useful for blocking PD-1 mediated interactions.

Other Names: PD-L2, PDL2, B7DC, CD273
Structure: B7 Immunoglobulin superfamily, 42 kD
Distribution: Dendritic cells, liver, few transformed cell lines, subset of macrophages; not expressed on lymphoid tissues
Function: Binds to PD-1 and alternative receptor; ligation on DC stimulates, inhibits T cell responses via PD-1 binding, stimulates T cells via alternative receptor binding and promotes tumor immunity
Ligand Receptor: PD-1, uncharacterized receptor
Antigen References:

1. Shin T, et al. 2003. J. Exp. Med. 198:31.
2. Liu X, et al. 2003. J. Exp. Med. 197:1721.
3. Carreno BM, et al. 2002. Annu. Rev. Immunol. 20:29.