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Safety Data Sheet (SDS) PE Anti-mouse CD275 B7-H2 B7-RP1 ICOS Ligand Antibody     Product Data Sheet (PDF)    
PE Anti-mouse CD275 (B7-H2, B7-RP1, ICOS Ligand) Antibody
1137025 50 µg $105.00       
Clone: HK5.3
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: Mouse B7-RP1 transfected cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: in vitro and in vivo blocking of ligand binding1,2. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody or biotinylated anti-rat IgG second step , followed by SAv-PE ).

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Iwai H, et al. 2003. J. Immunol. 171:2848. (Block)
2. Kohyama M, et al. 2004. P. Natl. Acad. Sci. USA 101:4192. (Block)
3. Zhang P, et al. 2007. J.Immunol. 178:474. PubMed
4. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed

C57Bl/6 mouse splenocytes stained with

C57Bl/6 mouse splenocytes stained with CD275 (clone HK5.3) PE



Description:

CD275 is a 40 kD protein, also known as ICOS ligand, B7-RP1, B7-H2, and B7h. It is a member of the immunoglobulin receptor superfamily expressed on splenic B cells, dendritic cells, and macrophages. The expression of this antigen is increased by TNF-α stimulation and is highly upregulated by local inflammation. B7RP1 binds to ICOS and co-stimulates T cell and B cell responses. Two isoforms of this protein have been reported to occur as a result of alternative splicing. These isoforms show differential tissue distribution.

Other Names: ICOS ligand, ICOSL, B7 homolog 2, B7-H2, B7H2, B7RP1, B7h, ICOS-L
Structure: B7 Immunoglobulin superfamily, approximately 40 kD
Distribution: Isoform 1 has highest expression in lymphoid tissues, splenic B-cells, T-cells, dendritic cells and macrophages; lower levels in many non-lymphoid tissues. The expression of isoform 2 is restricted to heart, spleen, and kidney.
Function: Binds to ICOS. Co-stimulates T cell responses including proliferation and cytokine secretion. Stimulates B cell proliferation and differentiation to plasma cells.
Ligand Receptor: ICOS
Antigen References:

1. Yoshinaga SK, et al. 1999. Nature 402:827.
2. Ling V, et al. 2000. J. Immunol. 164:1653.
3. Ling V, et al. 2001. J. Immunol. 164:1653.