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Safety Data Sheet (SDS) Biotin Anti-mouse CD253 TRAIL Antibody     Product Data Sheet (PDF)    
Biotin Anti-mouse CD253 (TRAIL) Antibody
1146515 50 µg $100.00       
Clone: N2B2
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: Mouse TRAIL-transfected 2PK-3 cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: in vitro blocking of NK cell cytotoxicity1,2.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Kayagaki N, et al. 1999. J. Immunol. 163:1906. (Block)
2. Sato K, et al. 2005. J. Immunol. 174:4025. (Block)
3. Joshi PS, et al. 2006. J. Leukocyte Biol. 80:1345.
4. Herold S, et al. 2008. J. Exp. Med. 205:3065. PubMed
5. Iannello A, et al. 2009. J. Virol. 83:5999. PubMed
6. Komatsu M, et al. 2003. Blood 101:3991. (Block)
7. Taieb J, et al. 2006. Nature Med. 12:214. (Block)
8. Lee SW, et al. 2013. Biochem Biophys Res Commun.291:1999. PubMed

Mouse TRAIL transfected cells stained

Mouse TRAIL transfected cells stained with biotinylated N2B2, followed by Sav-PE



Description:

CD253 is a 40 kD TNF superfamily member known as TRAIL, Apo-2 ligand, and Apo-2L. TRAIL is expressed on a variety of cells, including IL-2 and IL-15 activated NK cells and activated T cells. However, it is undetectable on resting T and B cells. TRAIL has been reported to induce apoptosis in tumor and transformed cell lines by a caspase-dependent process. The N2B2 antibody has been reported to be useful for flow cytometric staining and blocking NK cell cytotoxicity in vitro.

Other Names: TNFSF10, TNF-related apoptosis inducing ligand, Apo-2 ligand, Apo-2L, CD253
Structure: TNF ligand superfamily member, approximately 40 kD
Distribution: Widespread, highest expression in spleen, lung, prostate; NK, monocytes, neutrophils, activated T cells
Function: Induces apoptosis in tumorigenic and transformed cell lines, involved in T cell mediated cytotoxicity
Ligand Receptor: TRAIL receptor DR5
Antigen References:

1. Kayagaki N, et al. 1999. J. Immunol. 163:1906.
2. Wiley SR, et al. 1995. Immunity 3:673.
3. Wu GS, et al. 1999. Cancer Res. 59:2770.
4. Mariani SM, et al. 1998. Eur. J. Immunol. 28:973.