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Safety Data Sheet (SDS) Biotin Anti-mouse CD34 Antibody     Product Data Sheet (PDF)    
Biotin Anti-mouse CD34 Antibody
1196515 50 µg $80.00       
1196520 500 µg $270.00       
Clone: MEC14.7
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: Cells transfected with mouse CD34
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application:

FC - Quality tested

Application Notes:

The MEC14.7 antibody does not stain bone marrow cells like some other mouse CD34 antibodies, probably because the antibody recognizes a different epitope from other mAbs. Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting6, and immunohistochemistry of acetone-fixed frozen sections and paraffin-embedded sections2,4,5,6.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Garlanda C, et al. 1997. Eur. J. Cell Biol. 73:368. (FC)
2. Knowles HJ, et al. 2004. Circ. Res. 95:162. (IHC)
3. Trempus CS, et al. 2003. J. Invest. Dermatol. 120:501.
4. Winding B, et al. 2002. Clin. Cancer Res. 8:1932. (IHC)
5. Voswinckel R, et al. 2003. Circ. Res. 93:372. (IHC)
6. Kairaitis LK, et al. 2005. Am. J. Physiol. Renal. Physiol. 288:F198. (IHC, WB)
7. Ao A, et al. 2008. P. Natl. Acad. Sci. USA 105:7821. PubMed

Mouse NIH/3T3 cell line stained

Mouse NIH/3T3 cell line stained with biotinylated MEC14.7, followed by Sav-PE



Description:

CD34 is a highly glycosylated hematopoietic progenitor antigen. Two isoforms of CD34 have been reported to be generated by alternative splicing. This antigen is expressed on hematopoietic progenitors as well as on endothelial cells, brain, and testis. CD34 is thought to function as an adhesion molecule for early hematopoietic progenitors mediating the attachment of stem cells to extracellular matrix or stromal cells. CD34 is phosphorylated on serine residues by PKC.

Other Names: Mucosialin
Structure: Type I membrane protein, 75-120 kD, highly glycosylated; two isoforms reported
Distribution: Hematopoietic progenitors, brain, testis, endothelial cells; low expression in thymus, spleen, and bone marrow
Function: Possible adhesion molecule thought to function in early hematopoiesis by mediating attachment of stem cells to bone marrow extracellular matrix or stromal cells. Presents carbohydrate ligands to selectins.
Ligand Receptor: L-selectin, other selectins
Antigen References:

1. Garlanda C, et al. 1997. Eur. J. Cell Biol. 73:368.
2. Brown J, et al. 1991. Int. Immunol. 3:175.
3. Suda J, et al. 1992. Blood 79:2288.
4. Baumhueter S, et al. 1994. Blood 84:2554.