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Safety Data Sheet (SDS) Purified Anti-human/mouse CD49f Antibody     Product Data Sheet (PDF)    
Purified Anti-human/mouse CD49f Antibody
2168010 100 µg $95.00       
Clone: GoH3
Isotype: Rat IgG2a, κ
Reactivity: Human, Mouse, Cross-Reactivity: Baboon, Chimpanzee, Capuchin Monkey, Cynomolgus, Rhesus, Horse (Equine), Cattle (Bovine, Cow), Sheep (Ovine), Swine (Pig, Porcine), Dog (Canine), Cat (Feline), Rabbit (Lapine)
Immunogen: Mouse mammary tumor cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application:

FC - Quality tested
IF - Validated
IHC, IP - Reported in the literature

Application Notes:

Additional reported applications (for the relevant formats) include: immunoprecipitation1,5, in vitro and in vivo blocking of cell binding to laminin and blocking the function of integrin α61,4, and immunohistochemistry of acetone-fixed frozen sections2,3,5. The GoH3 antibody has been reported to block laminin binding in vitro and to block integrin α6 function in vivo.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Georas SN, et al. 1993. Blood 82:2872. (IP, Block)
2. Honda T, et al. 1995. J. Clin. Endocrinol. Metab. 80:2899. (IHC)
3. Sonnenberg A, et al. 1986. J. Histochem. Cytochem. 34:1037. (IHC)
4. Nakamura K, et al. 1997 Biochem. Biophys. Res. Commun. 235:524. (Block)
5. Sonnenberg A, et al. 1987 J. Biol. Chem. 262:10376. (IP, IHC)
6. Deregibus MC, et al. 2007. Blood doi:10.1182/blood-2007-03-078709.
7. Horwitz KB, et al. 2008. Proc Natl Acad Sci USA. 105:5774. PubMed
8. Nardella C, et al. 2009. Sci Signal. 2:55. PubMed
9. Xu T, et al. 2010. Mol Cancer Ther. 9:438. PubMed
10. Stepp MA, et al. 2007. J Cell Sci. 120:2851. PubMed
11. Jo M, et al. 2010. Cancer Res. 70:8948. PubMed
12. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
13. Grange C, et al. 2011. Cancer Res. 71:5346. PubMed
14. Lai KP, et al. 2012. Mol Endocrinol. 26:52. PubMed
15. Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. (FC) PubMed

Human peripheral blood lymphocytes stained

Human peripheral blood lymphocytes stained with purified GoH3, followed by anti-rat IgGs FITC

MDA-MB-231 breast cancer cells were

MDA-MB-231 breast cancer cells were stained with anti-CD49f (clone GoH3) followed by DyLight™ 649 Goat anti-rat Ig secondary antibody (red), plus DAPI staining for nuclei (blue). Images were taken under 20x bin4 (Filter set: EX647/10x, Dichroic 665LP, EM 700/70x) at exposure 4s. Data provided by Er Liu and John Nolan, La Jolla Institute for Bioengineering.



Description:

CD49f is a 120 kD integrin family member also known as VLA-6 α chain and α6 integrin subunit. CD49f associates with either integrin β1 (CD29) or integrin β4 (CD104) to form receptors (VLA-6 or α6β4 complex) for laminin and kalinin. CD49f is expressed on platelets, monocytes, T cells, placental trophoblasts, and epithelial and endothelial cells. CD49f is involved in adhesion and can act as a co-stimulatory molecule for T cell activation and proliferation.

Other Names: VLA-6 α chain, α6 integrin, integrin α6, ITGA6
Structure: Integrin family, associates with β1 or β4, 120 kD
Distribution: Platelets, monocytes, T cells, placental trophoblasts, epithelial and endothelial cells
Function: Adhesion, receptor for laminin and kalinin; laminin binding to VLA-6 induces T cell co-stimulation for proliferation and activation
Ligand Receptor: With integrin β1 (CD29) forms VLA-6, with integrin β4 (CD104) forms a6β4 integrin; laminin and kalinin are ligands for these receptors
Antigen References:

1. Sonnenberg A, et al. 1990. J. Cell Biol. 110:2145.
2. Sonnenberg A, et al. 1990. J. Cell. Sci. 96:207.
3. Aumailley M, et al. 1990. Exp. Cell Res. 188:55.
4. Niessen CM, et al. 1994. Exp. Cell Res. 211:360.