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Safety Data Sheet (SDS) Biotin Anti-mouse CD365 Tim-1 Antibody     Product Data Sheet (PDF)    
Biotin Anti-mouse CD365 (Tim-1) Antibody
1197515 50 µg $95.00       
Clone: RMT1-4
Isotype: Rat IgG2b, κ
Reactivity: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance in each application.

Application References:

1. Meyers JH, et al. 2005. Nat. Immunol. 6:455.
2. Nakae S, et al. 2007. Blood doi:10.1182/blood-2006-11-058800.
3. Yamanishi Y, et. al. 2010. J. Exp. Med. 207:1501. (FC) 
4. Namavari A, et al. 2012. Invest Ophthalmol Vis Sci. 53:4575. PubMed.

MouseTim-1 transfected cells stained with

MouseTim-1 transfected cells stained with biotinylated anti-mouse CD365 (Tim-1, clone RMT1-4), followed by Sav-PE



Description:

CD365 (Tim-1) is a transmembrane protein also known as T cell immunoglobulin and mucin domain containing protein-1 and hepatitis virus cellular receptor 1. It is developmentally expressed at high levels in the blastocyst. Tim-1 is expressed on activated CD4+ lymphocytes especially on Th2 cells and has been implicated to play a critical role in the development of atopic disease and other Th2-biased immune responses. Tim-1 is hepatitis A virus receptor in humans. Tim-4 is the endogenous ligand of Tim-1. The interaction of Tim-1 and Tim-4 is involved in costimulation of T cell proliferation.  Tim-1 is an endogenous ligand for LMIR5/CD300b.

Other Names: T cell immunoglobulin and mucin domain containing protein-1, T cell and airway phenotype regulator (Tapr), hepatitic virus cellular receptor 1, CD365
Structure: Transmembrane protein containing immunoglobulin domain and mucin-like domain; predicted molecular weight 33 kD.
Distribution: Developmentally regulated; highly expressed in embryonic development in blastocysts. Expressed on activated CD4+ lymphocytes, especially Th2 cell lines.
Function: Binds hepatitis virus, may play a critical role in immune responses and the development of atopic diseases (in particular airway hyperreactivity).
Ligand Receptor: Binds hepatitis A virus in humans, Tim-4, LMIR5/CD300b
Antigen References:

1. McIntire JJ, et al. 2001. Nature Immunol. 2:1109.
2. Kuchroo VK, et al. 2003. Nat. Rev. Immunol. 3:454.
3. Wills-Karp M, et al. 2001. Nat. Rev. Immunol. 1:69.
4. Meyers JH, et al. 2005. Nat. Immunol. 6(5):455.