The antibody was purified by affinity chromatography.
Storage & Handling:
The antibody solution should be stored undiluted between 2°C and 8°C.
FC - Quality tested IHC - Reported in the literature
Additional reported applications (for relevant formats) include: in vitro1 and in vivo2 blocking of Tim-3, and immunohistochemical staining of frozen sections2.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
1. Nakae S, et al. 2007. Blood 110(7):2565-8. (FC, Block) 2. Oikawa T, et al. 2006. J. Immunol. 177(7):4281-7. (FC, Block, IHC)
Mouse Tim-3 transfected cells stained with purified anti-mouse CD366 (Tim-3, clone RMT3-23), followed by anti-rat IgG FITC
CD366 (Tim-3) is a transmembrane protein also known as T cell immunoglobulin and mucin domain containing protein-3. Tim-3 is expressed at high levels on Th1 lymphocytes and CD11b+ macrophages. Tim-3 has also been shown to exist as a soluble protein. Cells expressing Tim-3 are present at high levels in the CNS of animals at the onset of experimental autoimmune encephalomyelitis (EAE), a disease mediated by lymphocytes secreting Th1-like cytokines. Tim-3 has been proposed to inhibit Th1-mediated immune responses and promote immunological tolerance.
T cell immunoglobulin and mucin domain containing 3 protein, hepatitis virus cellular receptor 2, CD366
Transmembrane protein containing immunoglobulin domain and mucin-like domain; predicted molecular weight 31 kD; can exist as a soluble form lacking mucin and transmembrane domains
Expressed on activated Th1 lymphocytes and CD11b+ macrophages
May play a role in the development of immune responses and the development of Th1-mediated responses
Putative ligand on resting CD4+ lymphocytes
1. Sabatos CA, et al. 2003. Nat. Immunol. 4:1102. 2. Sanchez-Fueyo A, et al. 2003. Nat. Immunol. 4:1102. 3. Kuchroo VK, et al. 2003. Nat. Rev. Immunol. 3:454. 4. Mooney L, et al. 2002. Nature 415:536.
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