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Safety Data Sheet (SDS) PE Anti-mouse CD265 RANK Antibody     Product Data Sheet (PDF)    
PE Anti-mouse CD265 (RANK) Antibody
1199025 50 µg $105.00       
1199030 200 µg $330.00       
Clone: R12-31
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance in each application.

Application References:

1. Hildebrand D, et al. 2010. Infect. Immun. 79:220. PubMed
2. Holoshitz J, et al. 2013. J. Immunol. 190:48. PubMed
3. Xia WF, et al. 2013. J Cell Biol. 200:821. PubMed
4. Adamopoulos IE, et al. 2015. Ann Rheum Dis. 74:1284. PubMed

Mouse RANK transfected cells stained

Mouse RANK transfected cells stained with R12-31 PE



Description:

CD265 is a type I transmembrane protein also known as receptor activator of NF-κB (RANK), tumor necrosis factor receptor superfamily member 11A (TNFRSF11A), and osteoclast differentiation factor receptor (ODFR). RANK is a receptor for RANK ligand/TRANCE/OPGL and is involved in osteoclastogenesis and T cell/dendritic cell interactions. RANK is ubiquitously expressed in trabecular bone, thymus, small intestine, lung, brain, and kidney.

Other Names: Receptor activator of NF-κB, tumor necrosis factor receptor superfamily member 11A (TNFRSF11A), osteoclast differentiation factor receptor (ODFR), CD265
Structure: TNF superfamily member, Type I membrane protein
Distribution: Ubiquitous expression; highest levels in trabecular bone, thymus, small intestine, lung, brain, and kidney. Weakly expressed in spleen and bone marrow.
Function: Essential for RANK ligand mediated osteoclastogenesis. Involved in the interactions between T cells and dendritic cells
Ligand Receptor: Receptor for RANK ligand/TRANCE/OPGL
Interaction: Cytoplasmic domain interacts with TRAF2, TRAF5, and TRAF6
Antigen References:

1. Anderson DM, et al. 1997. Nature 390:175.
2. Nakagawa N, et al. 1998. Biochem. Biophys. Res. Commun. 253:395.
3. Ha J, et al. 2010. J. Immunol. 184:4717.