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Safety Data Sheet (SDS) Purified Anti-human/mouse CD266 Fn14 TWEAK R Antibody     Product Data Sheet (PDF)    
Purified Anti-human/mouse CD266 (Fn14, TWEAK R) Antibody
2170510 100 µg $190.00       
Clone: ITEM-4
Isotype: Mouse IgG2b, κ
Reactivity: Human, Mouse
Immunogen: Human Fn14 transfected P815
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application:

FC - Quality tested
IF, IHC, WB - Reported in the literature

Application Notes:

Additional reported applications (for the relevant formats) include: Western blotting3,4, immunofluorescence3,4, and immunohistochemistry of frozen sections5 and formalin-fixed paraffin-embedded sections3. ITEM-4 antibody has no effect on TWEAK-induced MAPK activation in RAW cells1.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Polek TC, et al. 2003. J. Biol. Chem. 278:32317.
2. Nakayama M, et al. 2003. J. Immunol. 170:341.
3. Karaca G, et al. 2014. PLoS One. 9:83987. (IHC, IF, WB) PubMed
4. Muñoz-García B, et al. 2006. Stroke. 37:2044. (IF, WB)
5. Jakubowski A, et al. 2005. J. Clin. Invest. 115:2955. (IHC)
6. Karaca G, et al. 2015. Am J Physiol Gastrointest Liver Physiol. 308:325. PubMed

Human Fn14 transfected cells stained

Human Fn14 transfected cells stained with purified ITEM-4, followed by anti-mouse IgG FITC



Description:

CD266 is a 14 kD transmembrane protein known as TWEAK receptor (TWEAK-R) or fibroblast growth factor inducible 14 (Fn14). It is member 12A of the tumor necrosis factor receptor superfamily (TNFRSF12A) expressed at low levels in a variety of tissues, such as heart, placenta, lung, muscle, and pancreas. TWEAK-R binds TWEAK (CD255) and interacts with the cytoplasmic proteins TRAF1, 2, and 3. The engagement of Fn14 can induce cell death and may play a role in inflammation, hepatocyte growth, and liver neoplasia.

Other Names: Fibroblast growth factor-inducible 14 kD protein (Fn14), TWEAK receptor, TWEAK-R, TNFRSF12A
Structure: Transmembrane TNF superfamily receptor, contains no death domain, 14 kD
Distribution: Widely expressed in heart, placenta, lung, muscle, kidney, pancreas, and HUVEC
Function: Binds to TWEAK (CD255), thought to play a role in cell death and inflammation
Ligand Receptor: TWEAK (CD255); interacts with cytoplasmic proteins TRAF1, 2, and 3
Antigen References:

1. Feng SL, et al. 2000. Am. J. Path. 156:1253.
2. Meighan-Mantha RL, et al. 1999. J. Biol. Chem. 274:33166.
3. Nakayama M, et al. 2003. J. Immunol. 170:341.
4. Lynch CN, et al. 1999. J. Biol. Chem. 274:8455.