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Safety Data Sheet (SDS) Biotin Anti-mouse CD262 DR5 TRAIL-R2 Antibody     Product Data Sheet (PDF)    
Biotin Anti-mouse CD262 (DR5, TRAIL-R2) Antibody
1199520 500 µg $345.00       
Clone: MD5-1
Isotype: Armenian Hamster IgG
Reactivity: Mouse
Immunogen: Mouse DR5-Ig fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: in vitro and in vivo induction of cytotoxicity2,3.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Hayakawa Y, et al. 2004. J. Immunol. 172:123. (FC)
2. Takeda K, et al. 2004. J. Exp. Med. 199:437. (Cyt)
3. Sato K, et al. 2005. J. Immunol. 174:4025. (Cyt)
4. Iannello A, et al. 2009. J. Virol. 83:5999. PubMed
5. Grisendi G, et al. 2010. Cancer Res. 70:3718. PubMed



Description:

CD262 is a 55 kD protein also known as DR5, TRAIL-R2, Apo-2, TRICK2, and KILLER. DR5 is member 10B of the TNF receptor superfamily (TNFRSF10B), widely expressed in normal tissues and some tumor cell lines. The DR5 receptor binds to TRAIL and interacts with the cytoplasmic proteins TRADD and RIP. TRAIL binding to DR5 activates NF- κB and induces apoptosis. DR5 is induced by wild-type p53.

Other Names: DR5, TRAIL-R2, TNFRS10B, TRICK2, KILLER
Structure: Type I transmembrane protein, TNF receptor superfamily member, homotrimer, predicted molecular weight 55 kD
Distribution: Widely expressed in normal tissues and some tumor cell lines. Expression regulated by the tumor suppressor p53
Function: Binds to TRAIL, activates NF- ?B, induces apoptosis. May be involved in the regulation of tumor metastases.
Ligand Receptor: TRAIL (CD253); cytoplasmic domain interacts with TRADD and RIP
Antigen References:

1. Wu GS, et al. 1999. Cancer Res. 59:2770.