The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions.
Storage & Handling:
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
Additional reported applications (for relevant formats of this clone) include: immunoprecipitation1,4, in vitro blocking3,9,12, depletion2,8, immunofluorescence microscopy6,7,10, and immunohistochemistry of acetone-fixed frozen sections5,11-13 and paraffin sections28.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.
1. Springer T, et al. 1978. Eur. J. Immunol. 8:539. (IP) 2. Ault K and Springer T. 1981. J. Immunol. 126:359. (Deplete) 3. Springer TA, et al. 1982. Immunol. Rev. 68:171. (Block) 4. Ho MK and Springer TA. 1983. J. Biol. Chem. 258:2766. (IP) 5. Flotte TJ, et al. 1983. Am. J. Pathol. 111:112. (IHC) 6. Noel GJ, et al. 1990. J. Clin. Invest. 85:208. (IF) 7. Allen LA and Aderem A. 1996. J. Exp. Med. 184:627 (IF) 8. D'Amico A and Wu L. 2003. J. Exp. Med. 198:293. (Deplete) 9. Brickson SJ, et al. 2003. Appl Physiol. 95:969. (Block) 10. Clatworthy MR and Smith KG. 2004. J. Exp. Med. 199:717. (IF) 11. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC) 12. Zhang Y, et al. 2002. J. Immunol. 168:3088. (IHC) 13. Iwasaki A and Kelsall BL. 2001. J. Immunol. 166:4884 (IHC, FC) 14. Tailleux L. 2003. J. Exp. Med. 197:121. (Block, FC) 15. Olver S, et al. 2006. Cancer Research 66:571. (FC) 16. Tan SL, et al. 2006. J. Immunol. 176:2872. (FC) PubMed 17. Ponomarev ED, et al. 2006. J. Immunol. 176:1402. (FC) 18. Dzhagalov I, et al. 2007. Blood 109:1620. (FC) 19. Fazilleau N, et al. 2007. Nature Immunol. 8:753. 20. Rasmussen JW, et al. 2006. Infect. Immun.74:6590. PubMed 21. Napimoga MH, et al. 2008. J. Immunol. 180:609. PubMed 22. Elqaraz-Carmon V, et al. 2008. J. Lipid. Res. 49:1894. PubMed 23. Kim DD, et al. 2008. Blood 112:1109. PubMed 24. Guo Y, et al. 2008. Blood 112:480. PubMed 25. Norian LA, et al. 2009. Cancer Res. 69:3086. (FC) PubMed 26. Baumgartner CK, et al. 2010. J. Immunol. 184:573. PubMed 27. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed 28. Whiteland J, et al. 1995. J. Histochem. Cytochem. 43:313. (IHC) 29. Richardson ET, et al. 2015. Infect Immun. 83:2242. PubMed
C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) Alexa Fluor® 647 or rat IgG2b, κ Alexa Fluor® 647 isotype control (gated on total cells).
CD11b is a 170 kD glycoprotein also known as αM integrin, Mac-1 α subunit, Mol, CR3, and Ly-40. CD11b is a member of the integrin family, primarily expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b non-covalently associates with CD18 (β2 integrin) to form Mac-1. Mac-1 plays an important role in cell-cell interaction by binding its ligands ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, and fibrinogen.
αM integrin, Mac-1, Mo1, CR3, Ly-40, C3biR, ITGAM
Integrin family, associates with integrin β2 (CD18), 170 kD
Granulocytes, monocytes/macrophages, dendritic cells, NK cells, subsets of T and B cells
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Springer TA. 1994. Cell 76:301. 3. Coxon A, et al. 1996. Immunity 5:653.
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