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Safety Data Sheet (SDS) Alexa Fluor 488 Anti-mouse IL-2 Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 488 Anti-mouse IL-2 Antibody
3119065 100 µg $220.00       
Clone: JES6-5H4
Isotype: Rat IgG2b, κ
Reactivity: Mouse
Immunogen: E. coli-expressed, recombinant mouse IL-2
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

ICFC - Quality tested

Application Notes:

ELISA Detection1-3 or ELISPOT Detection4-6: The biotinylated JES6-5H4 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with the purified JES6-1A12 antibody as capture antibody and recombinant mouse IL-2 as the standard.
Flow Cytometry8-10: The fluorochrome-labeled JES6-5H4 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-2 -producing cells within mixed cell populations.
Neutralization1,7:

Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Application References:

1. Abrams J, et al. 1992. Immunol. Rev. 127:5.
2. Sander B, et al. 1993. J. Immunol. Meth. 166:201.
3. Abrams J. 1995. Curr. Prot. Immunol. John Wiley and Sons New York. Unit 6.20.
4. Klinman D, et al. 1994. Curr. Prot. Immunol. John Wiley and Sons New York. Unit 6.19.
5. Mo X, et al. 1995. J. Virol. 69:1288.
6. Karulin A, et al. 2000. J. Immunol. 164:1862.
7. Finkelman F, et al. 2003. Curr. Prot. Immunol. John Wiley & Sons New York. Unit 6.28.
8. Ko SY, et al. 2005. J. Immunol. 175:3309. PubMed
9. Kang SS and Allen PM. 2005. J. Immunol. 174:5382.
10. Lawson BR, et al. 2007. J. Immunol. 178:5366.

PMA+ionomycin-stimulated (6 hours) Balb/c mouse

PMA+ionomycin-stimulated (6 hours) Balb/c mouse splenocytes intracellularly stained with CD3 (17A2) PE and JES6-5H4 Alexa Fluor® 488



Description:

IL-2 is a potent lymphoid cell growth factor which exerts its biological activity primarily on T cells. Additionally, IL-2 has been found to stimulate growth and differentiation of B cells, NK cells, LAK cells, monocytes, and oligodendrocytes.

Other Names: Interleukin-2, T cell growth factor (TCGF), Eosinophil differentiation factor (EDF), Killer cell helper factor (KHF), Macrophage-activating factor for cytotoxicity I (MAF-C I), Thymocyte differentiation factor (TDF)
Structure: Cytokine; 15-30 kD (Mammalian)
Regulation: Upregulated by NFAT; downregulated by TCF-8, CIF (colostrum inhibitory factor)
Cellular Sources: T cells
Cellular Targets: T cells, B cells, NK cells, LAK cells, monocytes, macrophages, oligodendrocytes
Receptors: High affinity heterotrimer of IL-2Rα/β/γ, intermediate affinity homodimer IL-2Rα (CD25; p55; Tac) and heterodimer IL-2Rβ (CD122)/γ; γ-subunit (CD132) in common with IL-4R, IL-7R, IL-13R, IL-15R
Bioactivity/Activities: Proliferation of T lymphocytes, B cells, anti-inflammatory, hematopoiesis, tumor surveillance
Antigen References:

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Taniguchi T, et al. 1993. Cell 73:5.
3. Nistico G. 1993. Prog. Neurobiol. 40:463.
4. Waldmann T, et al. 1993. Ann. NY Acad. Sci. 685:603.


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