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Safety Data Sheet (SDS) Alexa Fluor 647 Anti-mouse IL-10 Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 647 Anti-mouse IL-10 Antibody
3125080 25 µg $100.00       
3125070 100 µg $220.00       
Clone: JES5-16E3
Isotype: Rat IgG2b, κ
Reactivity: Mouse
Immunogen: E. coli-expressed, recombinant mouse IL-10
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

ICFC - Quality tested

Application Notes:

ELISA or ELISPOT Detection1,9,11: The biotinylated JES5-16E3 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified JES5-2A5 antibody as the capture antibody.

Neutralization14:

Recommended Usage:

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.

Application References:

1. Simkin G, et al. 2000. J. Immunol. 164:2457.
2. Kitagaki K, et al. 2002. Clin. Diagn. Lab Immunol. 9:1260.
3. Khanna A, et al. 2000. J. Immunol. 164:1346.
4. Sander B, et al. 1993. J. Immunol. Methods 166:201.
5. Litton M, et al. 1994. J. Immunol. Methods 175:47.
6. Andersson U, et al. 1999. Detection and qunatification of gene expression. New York:Springer-Verlag.
7. Finkelman F, et al. 2003. Curr. Prot. Immunol. John Wiley & Sons New York. Unit 6.28.
8. Wang W, et al. 2004. FASEB J. 18:1043.
9. Brummel R and Lenert P. 2005. J. Immunol. 174:2429.
10. Lawson BR, et al. 2007. J. Immunol. 178:5366.
11. Xu G, et al. 2007. J. Immunol. 179:5358. PubMed
12. Brummel R, et al. 2005. J. Immunol.174:2429. PubMed
13. Kang YJ, et al. 2007. Stem Cells 25:1814. PubMed
14. Seo N, et al. 2001. Immunology. 103:449. (Neut)

PMA/ionomycin-stimulated Th2-polarized Balb/c mouse splenocytes

PMA/ionomycin-stimulated Th2-polarized Balb/c mouse splenocytes were intracellular stained with JES5-16E3 Alexa Fluor® 647 and CD3 (17A2) PE



Description:

IL-10 was originally described as Cytokine Synthesis Inhibitory Factor (CSIF) by virtue of its ability to inhibit cytokine production by Th1 clones. IL-10 shares over 80% sequence homology with the Epstein-Barr virus protein BCRFI. IL-10 inhibits IFN-γ, TNF-β, and IL-2 production by Th1 clones; inhibits macrophage-mediated IL-1, IL-6, and TNF-α synthesis; suppresses the delayed type hypersensitivity response; stimulates Th2 cell response (which results in elevated antibody production); and promotes mast cell proliferation in combination with IL-4.

Other Names: Interleukin-10, Cytokine synthesis inhibitory factor (CSIF), B cell derived T cell growth factor (B-TCGF), T cell growth inhibitory factor (TGIF)
Structure: Acid-labile cytokine, dimer, 17-21 kD (Mammalian)
Regulation: Downregulated by IL-4, IL-10
Cellular Sources: Activated CD8+ T cells, Th0, Th2 subset of CD4+ T cells, Ly-1+ B cells, monocytes, macrophages, keratinocytes
Cellular Targets: T cells, B cells, mast cells, macrophages
Receptors: IL-10R (CDw210)
Antigen References:

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. de Waal-Malefy R, et al. 1992. Curr. Opin. Immunol. 4:314.
3. Howard M, et al. 1992. Immunol. Today 13:198.
4. Quesniaux V. 1992. Res. Immunol. 143:385.
5. Norton SK, et al. 2008. J. Immunol. 180:2848.


This product is provided under an agreement between Life Technologies Corporation and Sony Biotechnology Inc. and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications, and corresponding non-U.S. equivalents, owned by and/or licensed to Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research and/or development conducted by the buyer (whether the buyer is an academic or for profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to (1) use of the product or its components in manufacturing, (2) use of the product or its components for therapeutic, diagnostic or prophylactic purposes, or (3) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Molecular Probes Detection Technologies, Attn: Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.