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Safety Data Sheet (SDS) PE Anti-rat TCR Gamma/delta Antibody     Product Data Sheet (PDF)    
PE Anti-rat TCR γ/δ Antibody
1613025 50 µg $90.00       
Clone: V65
Isotype: Mouse IgG1, κ
Reactivity: Rat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Additional reported applications (for the relevant formats) include: immunoprecipitation, immunohistochemical stainning of acetone-fixed frozen sections, in vitro stimulation and in vivo depletion.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Kuhnlein P, et al. 1994. J. Immunol. 153:979.
2. Pelegri C, et al. 1996. Arthritis Rheum. 39(2):204.
3. Tsuchiya T, et al. 2002. Amer. J. of Respir. and Crit. Care Med. 165:1640.
4. Hoffman MH, et al. 2012. Ann Rheum Dis. 72:1239. PubMed

LOU rat splenocytes stained with

LOU rat splenocytes stained with CD3 (1F4) APC and V65 PE



Description:

TCR γδ expression is observed on a subset of cells in the thymus, intestinal epithelium, skin, liver, peripheral lymphoid tissues, and peritoneum. The TCR γδ is involved in the antigen recognition of some bacterial or tumor-associated antigens presented by MHC class I. Immobilized V65 antibody has been reported to activate TCR-γδ -bearing T cells in vitro, and to deplete peripheral TCR-γδ -bearing T cells in vivo

.

Other Names: TCR-γ/δ, γ/δ TCR
Structure: Ig superfamily, associates with CD3 complex
Distribution: T subset in thymus, intestinal epithelium, peripheral lymphoid tissues and peritoneum
Function: Antigen recognition
Ligand Receptor: Some bacterial or tumor antigens bound MHC class I
Antigen References:

1. Allison TJ, et al. 2001. Nature 411:820.
2. Chien YH, et al. 1996. Ann. Rev. Immunol.14:511.
3. Hiromatsu K, et al. 1992. J. Exp. Med. 175:49.
4. Bluestone JA, et al. 1991. Immunol. Rev. 120:5.