The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance in each application.
1. Meyers JH, et al. 2005. Nat. Immunol. 6:455. 2. Nakae S, et al. 2007. Blood doi:10.1182/blood-2006-11-058800. 3. Yamanishi Y, et. al. 2010. J. Exp. Med. 207:1501. (FC) 4. Namavari A, et al. 2012. Invest Ophthalmol Vis Sci. 53:4575. PubMed.
CD365 (Tim-1) is a transmembrane protein also known as T cell immunoglobulin and mucin domain containing protein-1 and hepatitis virus cellular receptor 1. It is developmentally expressed at high levels in the blastocyst. Tim-1 is expressed on activated CD4+ lymphocytes especially on Th2 cells and has been implicated to play a critical role in the development of atopic disease and other Th2-biased immune responses. Tim-1 is hepatitis A virus receptor in humans. Tim-4 is the endogenous ligand of Tim-1. The interaction of Tim-1 and Tim-4 is involved in costimulation of T cell proliferation. Tim-1 is an endogenous ligand for LMIR5/CD300b.
T cell immunoglobulin and mucin domain containing protein-1, T cell and airway phenotype regulator (Tapr), hepatitic virus cellular receptor 1, CD365
Transmembrane protein containing immunoglobulin domain and mucin-like domain; predicted molecular weight 33 kD.
Developmentally regulated; highly expressed in embryonic development in blastocysts. Expressed on activated CD4+ lymphocytes, especially Th2 cell lines.
Binds hepatitis virus, may play a critical role in immune responses and the development of atopic diseases (in particular airway hyperreactivity).
Binds hepatitis A virus in humans, Tim-4, LMIR5/CD300b
1. McIntire JJ, et al. 2001. Nature Immunol. 2:1109. 2. Kuchroo VK, et al. 2003. Nat. Rev. Immunol. 3:454. 3. Wills-Karp M, et al. 2001. Nat. Rev. Immunol. 1:69. 4. Meyers JH, et al. 2005. Nat. Immunol. 6(5):455.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Sony, the Sony logo, Sony Biotechnology Inc. and logo, iCyt, the iCyt logo EC800 and SY3200 are trademarks of Sony Corporation. Alexa Fluor® and Pacific Blue™ are registered trademarks of and licensed under patents assigned to Life Technologies Corporation. Cy and CyDye are trademarks of GE Healthcare Limited. iCyt products containing Cy5, Cy5.5 or Cy7 are manufactured under license from GE Healthcare under U.S. Patent Numbers 5,569,587; 5,627,027 and patents or pending applications that are continuations, continuations-in-part, re-examinations, divisionals, reissues or foreign equivalents thereof. Brilliant Violet™, Brilliant Violet 421™, BV421™, Brilliant Violet 570™, BV570™, Brilliant Violet 605™, BV605™, Brilliant Violet 650™, and BV650™, Brilliant Violet 510™, BV510™, Brilliant Violet 711™, BV711™, Brilliant Violet 785™ and BV785™ are trademarks of Sirigen Group Ltd. PE-Dazzle 594™ is a trademark of Biolegend. All other trademarks are property of their respective owners. For Research Use Only. Not for use in diagnostic or therapeutic procedures.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.