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Safety Data Sheet (SDS) Alexa Fluor 488 Anti-mouse CD197 CCR7 Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 488 Anti-mouse CD197 (CCR7) Antibody
1200560 25 µg $120.00       
1200550 100 µg $290.00       
Clone: 4B12
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: Mouse CCR7 transfected RBL-2H3 cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

The 4B12 antibody does not inhibit binding of ligand to receptor. Additional reported applications (for the relevant formats) include: immunoprecipitation. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 is recommended prior to immunofluorescent staining.html" target="_blank">(see supplemental data of PE staining at differing temperatures).

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Application References:

1. Ohl L, et al. 2004. Immunity 21:279.
2. Ritter U, et al. 2004. J. Leukocyte Biol. 76:472.
3. Lan YY, et al. 2005. Am. J. Transplant. 5:2649. (FC)
4. Lee JH, et al. 2007. J. Immunol. 178:301. (FC) PubMed
5. Kurooka M and Kaneda Y. 2007. Cancer Res. 67:227. (FC) PubMed
6. Thompson BD. 2007. J. Biol. Chem. 282:9547. (FC)
7. Sakai N, et al. 2006. P. Natl. Acad. Sci. USA 103:14098. (FC)
8. Turnquist HR, et al. 2007. J. Immunol. 178:7018. (FC)
9. Hwang IY, et al. 2007. J. Immunol. 179:439. (FC) PubMed
10. Kang SG, et al. 2007. J. Immunol. 179:3724.
11. Mao A et al. 2005. J. Immunol. 175:5146. PubMed
12. Allende ML, et al. 2008. FASEB J. 22:307. PubMed
13. Kang SG, et al. 2007. J. Immunol. 179:3724. PubMed
14. Chen H, et al. 2005. J. Immunol. 175:591. PubMed
15. Florido M, et al. 2009. Immunobiology. 214:643. PubMed
16. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
17. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed

C57BL/6 splenocytes stained with 4B12

C57BL/6 splenocytes stained with 4B12 Alexa Fluor® 488 and CD3 (145-2C11) PE



Description:

CD197 is also known as C-C chemokine receptor 7 (CCR7) or EBI-1. CCR7 is a G-coupled rhodopsin-like member of the GPCR superfamily with a predicted molecular weight of 43 kD that is expressed on hematopoietic stem cells, most naive T cells, some memory T cells, B subset, and mature dendritic cells. CCR7 is a receptor for the chemokines CCL19 (MIP3 beta) and SLC (6CKine, Exodus-2, TCA-4, CCL21) that plays a role in thymocytes development, T cell adhesion at intestinal sites, the homeostatic recirculation of memory T cells, and chemotaxis.

Other Names: C-C chemokine receptor type 7 (CCR7), MIP-3 beta receptor, EBV-induced G protein coupled receptor 1, EBI-1, CD197
Structure: Rhodopsin-like GPCR superfamily, G-protein coupled receptor 1 family, integral membrane protein, predicted molecular weight 43 kD
Distribution: Hematopoietic stem cells, T subsets, mature dendritic cells
Function: Homozygous mutation. Receptor for MIP-3 beta and SLC chemokines. Probable mediator of EBV effects on B lymphocytes. Plays a role in T cell adhesion at intestinal sites; may also play a role in the homeostatic recirculation of memory T cells and chemotaxis.
Ligand Receptor: MIP3 beta, SLC (6CKine, Exodus-2, TCA-4)
Antigen References:

1. Schweickart VL, et al. 1994. Genomics 23:643.
2. Yoshida R, et al. 1998. J. Biol. Chem. 273:7118.
3. Campbell JJ, et al. 1998. J. Cell Biol. 141:1053.
4. Willimann K, et al. 1998. Eur. J. Immunol. 28:2025.


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