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Safety Data Sheet (SDS) Purified Anti-mouse CD40 Antibody     Product Data Sheet (PDF)    
Purified Anti-mouse CD40 Antibody
1114505 50 µg $65.00       
1114510 500 µg $190.00       
Clone: HM40-3
Isotype: Armenian Hamster IgM
Reactivity: Mouse
Immunogen: (BALB/c x NZB)F1 mouse-derived lymphoma WEHI 231
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: This antibody is at >85% purity.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application:

FC - Quality tested
IHC - Reported in the literature

Application Notes:

Additional reported applications (for the relevant formats) include: blocking of CD40 ligand binding1, stimulation of mouse B cell and dendritic cell proliferation2-6,9, and immunohistochemistry7 of acetone-fixed frozen sections.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Kawano T, et al. 1997. Science 278:1626. (Block)
2. Kaneko Y, et al. 1996. Eur. J. Immunol. 26:3061. (Costim)
3. Kashiwada M, et al. 1996. Eur. J. Immunol. 26:1451. (Costim)
4. Inaba M, et al. 1995. Eur. J. Immunol. 25:1244. (Costim)
5. Akiba H, et al. 1999. J. Immunol. 162:7058. (Costim)
6. Ridge JP, et al. 1998. Nature 393:474. (Costim)
7. Morelli AE, et al. 2000. Transplantation 69:2647. (IHC)
8. Ma XT, et al. 2006. Cancer Research 66:1169.
9. Yu X, et al. 2006. J. Biol. Chem. 281:15505. (Costim)
10. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
11. Folgosa L, et al. 2013. J. Immunol. 191:5951. PubMed

C57BL/6 splenocytes stained with HM40-3

C57BL/6 splenocytes stained with HM40-3 biotin, then detected with Sav-PE



Description:

CD40 is a 48 kD type I transmembrane glycoprotein, also known as Bp50. It is a member of the tumor necrosis factor receptor (TNFR) superfamily, expressed on B cells, basal epithelial cells, macrophages, follicular dendritic cells, endothelial cells, and a subset of CD34+ hematopoietic progenitors. CD40 regulates B cell development/maturation, Ig isotype switching and, in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with its ligand CD154 (gp39) expressed on activated T cells, is important in costimulation and immune regulation. The HM40-3 antibody has been reported to stimulate dendritic and B cell proliferation and block CD40-CD40L interactions.

Other Names: Bp50
Structure: TNFR superfamily, 48 kD
Distribution: B cells, basal epithelial cells, macrophages, follicular dendritic cells, endothelial cells, CD34+ hematopoietic progenitors
Function: Stimulates B cell growth, differentiation and isotype switching, stimulates macrophage and dendritic cell cytokine production
Ligand Receptor: CD40L (CD154)
Antigen References:

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Bancherou J, et al. 1994. Annu. Rev. Immunol. 12:881.
3. Clark EA, et al. 1996. P. Natl. Acad. Sci. USA 83:4494.