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Safety Data Sheet (SDS) FITC Anti-mouse CD48 Antibody     Product Data Sheet (PDF)    
FITC Anti-mouse CD48 Antibody
1117015 50 µg $95.00       
1117020 500 µg $255.00       
Clone: HM48-1
Isotype: Armenian Hamster IgG
Reactivity: Mouse
Immunogen: Mouse T lymphoma MBL-2
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

The HM48-1 antibody is useful for blocking in vitro and in vivo CD48 mediated interactions. Additional reported applications (for the relevant formats) include: immunoprecipitation1, costimulation of T cell proliferation1,2, blocking of CD48-CD2 interaction1, and inhibition of CTL activity and graft rejection1,2.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Kato K, et al. 1992. J. Exp. Med. 176:1241. (IP, Costim, Block)
2. Qin L, et al. 1994. J. Exp. Med. 179:341. (Costim, Block)
3. Theocharides AP, et al. 2012. J Exp Med. 209:1883. PubMed
4. Chou S, et al. 2013. Exp Hematol. 41:479. PubMed
5. Artinger EL, et al. 2013. PNAS. 110:12000. PubMed
6. Maltby S, et al. 2014. J Immunol. 193:4072. PubMed

C57BL/6 splenocytes stained with HM48-1

C57BL/6 splenocytes stained with HM48-1 FITC



Description:

CD48 is a 45 kD GPI-anchored glycoprotein also known as BCM1, Blast-1 (human), and OX-45 (rat). It is a member of the Ig superfamily, expressed on T and B cells and monocytes/macrophages. It plays a role in adhesion and T cell recognition. The primary ligands for CD48 are CD2 and CD244.

Other Names: BCM1, SLAMF2, Blast-1 (human), OX-45 (rat)
Structure: Ig superfamily, 45 kD
Distribution: T cells and B cells, monocytes/macrophages
Function: Adhesion, T cell recognition
Ligand Receptor: CD2, CD244
Antigen References:

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Flament C, et al. 1996. Hum. Immunol. 46:82.
3. Van der Merwe PA, et al. 1995. Curr. Biol. 5:74.
4. Latchman Y, et al. 1998. J. Immunol. 161:5809.