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Safety Data Sheet (SDS) Purified Anti-mouse GITR Ligand Antibody     Product Data Sheet (PDF)    
Purified Anti-mouse GITR Ligand Antibody
1201510 200 µg $245.00       
Clone: YGL 386
Isotype: Rat IgG1, κ
Reactivity: Mouse
Immunogen: Recombinant mouse GITRL protein and Transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application:

FC - Quality tested

Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References:

1. Tone M, et al. 2003. Proc. Natl. Acad. Sci. USA 100:15059
2. Lu LF, et al. 2005. J. Immunol. 175:1651
3. Esparza EM and RH Arch. 2005. J. Immunol. 174:7875
4. Ray A, et al. 2012. J. Immunol. 188:3188. PubMed.



Description:

GITR ligand (GITRL) is a member of the TNF superfamily, also known as TNFSF18 or TL6. It is expressed on B cells, macrophages, dendritic cells and endothelial cells. The expression of GITRL on dendritic cell surface can be transiently up-regulated and then rapidly down-regulated by LPS stimulation. GITRL binds to its receptor, GITR, to co-stimulate T cell activation, proliferation, and cytokine production, and inhibit the suppressive functions of CD4+CD25+ Treg cells, as well as induce macrophages activation. GITR-GITRL interaction has been implicated as an essential regulator of immune response to self antigens and phathogens.

Other Names: AITRL, TNFSF18, TL6, GITRL
Structure: TNF superfamily member
Distribution: B cells, macrophages, dendritic cells, endothelial cells.
Function: Bind GITR receptor to co-stimulate T cells activation, proliferation, and cytokine production; inhibit the suppressive activities of CD4+CD25+ Treg cells.
Ligand Receptor: GITR (TNFRSF18)
Antigen References:

1. Tone M, et al. 2003. Proc. Natl. Acad. Sci. USA 100:15059.
2. Kim JD, et al. 2003. Genes Immun. 4:564.
3. Yu KY, et al. 2003. Biochem. Biophys. Res. Commun. 310:433.
4. Shin HH, et al. 2002. Cytokine 19:187.