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Safety Data Sheet (SDS) Alexa Fluor 488 Anti-mouse CD357 GITR Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 488 Anti-mouse CD357 (GITR) Antibody
1201055 100 µg $290.00       
Clone: YGITR 765
Isotype: Rat IgG2b, κ
Reactivity: Mouse
Immunogen: Recombinant mouse GITR protein and Transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Application References:

1. Cobbold SP, et al. 2004. J. Immunol. 172:6003.
2. Biburger M, et al. 2008. J. Leukocyte Biol. 84:264. PubMed

BALB/c mouse splenocytes stained with

BALB/c mouse splenocytes stained with PE anti-muse CD25 (PC61) and Alexa Fluor® 488 rat IgG2b, k isotype control (bottom) or Alexa Fluor® 488 YGITR 765 (upper)





Description:

GITR, Glucocorticoid-induced TNFR-related gene, is a member of the TNF receptor superfamily, also known as TNFRSF18, and AITR (in humans). It is expressed at low levels on resting T lymphocytes and at high levels on CD4+CD25+ T regulatory (Treg) cells. The expression of GITR on T cells can be upregulated upon activation. Interaction of GITR with its ligand (GITRL) has been demonstrated to augment T cell activation, proliferation, cytokine production, as well as MAPKs and NF-κB activation, and abrogate the inhibitory functions of CD4+CD25+ Treg cells. In vivo activation GITR causes development of autoimmune diseases and restores the suppressed immune responses.

Other Names: TNFRSF18, AITR
Structure: TNF receptor superfamily member
Distribution: Low levels on resting T cells, high on CD4+CD25+ Treg cells, expression upregulated upon activation.
Function: Co-stimulate GITR expressing cells activation, proliferation, and cytokine production; inhibit the suppressive activities of CD4+CD25+ Treg cells.
Ligand Receptor: GITR ligand (TNFSF18)
Antigen References:

1. Tone M, et al. 2003. Proc. Natl. Acad. Sci. USA 100:15059
2. Ronchetti S, et al. 2004. Eur. J. Immunol. 34:613
3. Kanamaru F, et al. 2004. J. Immunol. 172:613
4. Shimizu J, et al. 2002. Nat. Immunol. 3:135
5. Stephens GL, et al. 2004. J. Immunol. 173:5008
6. McHugh RS, et al. 2002. Immunity 16:311


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