The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
The HM48-1 antibody is useful for blocking in vitro and in vivo CD48 mediated interactions. Additional reported applications (for the relevant formats) include: immunoprecipitation1, costimulation of T cell proliferation1,2, blocking of CD48-CD2 interaction1, and inhibition of CTL activity and graft rejection1,2.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
1. Kato K, et al. 1992. J. Exp. Med. 176:1241. (IP, Costim, Block) 2. Qin L, et al. 1994. J. Exp. Med. 179:341. (Costim, Block) 3. Liu J, et al. 2012. PLoS One. 7:e44044. PubMed 4. Revollo J, et al. 2015. Mutagenesis. 30:315. PubMed
C57BL/6 mouse splenocytes stained with HM48-1 PE
CD48 is a 45 kD GPI-anchored glycoprotein also known as BCM1, Blast-1 (human), and OX-45 (rat). It is a member of the Ig superfamily, expressed on T and B cells and monocytes/macrophages. It plays a role in adhesion and T cell recognition. The primary ligands for CD48 are CD2 and CD244.
BCM1, SLAMF2, Blast-1 (human), OX-45 (rat)
Ig superfamily, 45 kD
T cells and B cells, monocytes/macrophages
Adhesion, T cell recognition
1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Flament C, et al. 1996. Hum. Immunol. 46:82. 3. Van der Merwe PA, et al. 1995. Curr. Biol. 5:74. 4. Latchman Y, et al. 1998. J. Immunol. 161:5809.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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