Sony Biotechnology Inc. Sony Biotechnology Inc.cart
About Us Products What's New Library News Calendar Contact Us Place Order
Safety Data Sheet (SDS) Purified Anti-mouse CD48 Antibody     Product Data Sheet (PDF)    
Purified Anti-mouse CD48 Antibody
1117010 500 µg $215.00       
Clone: HM48-1
Isotype: Armenian Hamster IgG
Reactivity: Mouse
Immunogen: Mouse T lymphoma MBL-2
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application:

FC - Quality tested
IHC-F - Validated
IP - Reported in the literature

Application Notes:

The HM48-1 antibody is useful for blocking in vitro and in vivo CD48 mediated interactions. Additional reported applications (for the relevant formats) include: immunoprecipitation1, costimulation of T cell proliferation1,2, blocking of CD48-CD2 interaction1, and inhibition of CTL activity and graft rejection1,2.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. For immunohistochemical staining of frozen sections, a concentration range of 1.0 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Kato K, et al. 1992. J. Exp. Med. 176:1241. (IP, Costim, Block)
2. Qin L, et al. 1994. J. Exp. Med. 179:341. (Costim, Block)

C57BL/6 mouse splenocytes stained with

C57BL/6 mouse splenocytes stained with purified HM48-1, followed by anti-Armenian hamster IgG FITC.

C57BL/6 mouse frozen spleen section

C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% rat serum + FBS for one hour at room temperature. Then the section was stained with 5 µg/ml of purified CD48 (clone HM48-1) and DyLight™ 594 Goat anti-hamster (Armenian) IgG secondary antibody (red), and 5 µg/ml of B220 (clone RA3-6B2) Brilliant Violet 510™ (yellow) overnight at 4°C. The image was captured with a 10X objective.



Description:

CD48 is a 45 kD GPI-anchored glycoprotein also known as BCM1, Blast-1 (human), and OX-45 (rat). It is a member of the Ig superfamily, expressed on T and B cells and monocytes/macrophages. It plays a role in adhesion and T cell recognition. The primary ligands for CD48 are CD2 and CD244.

Other Names: BCM1, SLAMF2, Blast-1 (human), OX-45 (rat)
Structure: Ig superfamily, 45 kD
Distribution: T cells and B cells, monocytes/macrophages
Function: Adhesion, T cell recognition
Ligand Receptor: CD2, CD244
Antigen References:

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Flament C, et al. 1996. Hum. Immunol. 46:82.
3. Van der Merwe PA, et al. 1995. Curr. Biol. 5:74.
4. Latchman Y, et al. 1998. J. Immunol. 161:5809.