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Safety Data Sheet (SDS) PE Anti-human/mouse Integrin Beta7 Antibody     Product Data Sheet (PDF)    
PE Anti-human/mouse Integrin β7 Antibody
2206015 25 tests $95.00       
2206020 100 tests $215.00       
Clone: FIB504
Isotype: Rat IgG2a, κ
Workshop Number: VI 6T-101, VI A024
Reactivity: Human, Mouse, Cross-Reactivity: Chimpanzee, Baboon, Cynomolgus, Rhesus
Immunogen: TK1 cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

The FIB504 antibody has been reported to react with mouse and human β7 integrin and to block β7 integrin-mediated cell adhesion in in vitro and in vivo studies. Additional reported applications (for the relevant formats) include: blocking of cell adhesion1,3,4.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Andrew DP, et al. 1994. J. Immunol. 153:3847. (Block)
2. Berlin C, et al. 1993. Cell 74:185.
3. Rott LS, et al. 1996. J. Immunol. 156:3727. (Block)
4. Rivera-Nieves J, et al. 2005. J. Immunol. 174:2343. (Block)
5. Ohmori K, et al. 2009. J. Immunol. 182:2835. PubMed
6. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)

Human peripheral blood lymphocytes stained

Human peripheral blood lymphocytes stained with FIB504 PE

C57BL/6 mouse splenocytes stained with

C57BL/6 mouse splenocytes stained with FIB504 PE



Description:

Integrin β7 is a 130 kD glycoprotein also known as integin βp. It is a member of the Ig superfamily. In association with integrin α4 or αE chain, β7 forms α4/β7 or αE/β7 heterodimer. α4/β7 (CD49d/β7, LPAM-1) is expressed on the majority of peripheral lymphocytes, on small subsets of thymocytes, and bone marrow progenitors. LPAM-1 binds to several ligands, VCAM-1, MAdCAM-1 and fibronectin, and is involved in lymphocyte adhesion and some hematopoietic progenitor cells migration. αE/β7 (CD103/β7,αIEL/β7) is expressed on intestinal intraepithelial lymphocytes (IEL), dendritic epidermal T cells, T regulatory cells, a subset of CD8+ T cells in lymph nodes and lamina propria. CD103/β7 complex is thought to play a role in lymphocyte retention via interaction with its ligand E-Cadherin.

Other Names: β7 Integrin, integrin βp, ITGB7
Structure: Integrin family, Ig superfamily, 130 kD, heterodimer with CD49d or CD103
Distribution: CD49d/β7: majority of lymphocytes, thymocyte subset, and some bone marrow progenitors; CD103/β7: intestinal intraepithelial lymphocytes (IEL), dendritic epidermal T cells, subset of CD8 T cells in lymph nodes
Function: Lymphocyte adhesion, hematopoietic progenitor cells migration
Ligand Receptor: CD49d/β7: VCAM-1 (CD106), MAdCAM-1 and fibronectin; CD103/β7: E-cadherin
Antigen References:

1. Andrew DP, et al. 1994. J. Immunol. 153:3847.
2. Picarella D, et al. 1997. J. Immunol. 158:2099.
3. Lefrancois L, et al. 1994. Eur. J. Immunol. 24:635
4. Cepek KL, et al. 1994. Nautre 372:190.