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Safety Data Sheet (SDS) Alexa Fluor 488 Anti-mouse CD49b Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 488 Anti-mouse CD49b Antibody
1117550 25 µg $90.00       
Clone: HMα2
Isotype: Armenian Hamster IgG
Reactivity: Mouse
Immunogen: Mouse colon carcinoma cell line Colon26
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested
IF - Reported in the literature

Application Notes:

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunofluorescence4, and blocking of cell adhesion1-3.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Application References:

1. Miyake S, et al. 1994. Eur. J. Immunol. 24:2000. (Block, IP)
2. Noto K, et al. 1995. Int. Immunol. 7:835. (Block)
3. Arase H, et al. 2001. J. Immunol. 167:1141. (Block)
4. Zhang Z, et al. 2008. Blood 111:1980. (IF)

C57BL/6 mouse splenocytes stained with

C57BL/6 mouse splenocytes stained with HMα2 Alexa Fluor® 488 and DX5 PE



Description:

CD49b is a 150 kD glycoprotein, also known as α2 integrin, VLA-2 α chain, Integrin α2 chain, and HMα2. It is a member of the integrin family, expressed on NK cells, a subset of splenic CD4+ T cells, NK-T cells, intestinal intraepithelial and lamina propria lymphocytes, epithelial cells, and platelets. By associating with CD29 (integrin β1 subunit), CD49b forms the VLA-2 (integrin α2β1 ) complex. It plays a critical role in both adhesion and lymphocyte activation. The primary ligands for CD49b are collagen, laminin, and fibronectin. The HMα2 antibody has been shown to be useful for partially blocking CD49b mediated interactions with collagen. Additionally, this antibody blocks staining of splenic NK cells by the monoclonal antibody DX5.

Other Names: α2 integrin, VLA-2 α chain, Integrin α2 chain, ITGA2, HMa2
Structure: Integrin family, associates with CD29 (β1 integrin), 150 kD
Distribution: NK cells, subset splenic CD4+ T cells, NK-T cells, intestinal intraepithelial and lamina propria lymphocytes, epithelial cells, platelets
Function: Adhesion, lymphocyte activation
Ligand Receptor: Collagen, laminin, fibronectin
Antigen References:

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Sasaki K, et al. 2003. Int. Immunol. 15:701.
3. Inoue O, et al. 2003. J. Cell Biol. 160:769.


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