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Safety Data Sheet (SDS) FITC Anti-human/mouse Cutaneous Lymphocyte Antigen CLA Antibody     Product Data Sheet (PDF)    
FITC Anti-human/mouse Cutaneous Lymphocyte Antigen (CLA) Antibody
2206525 25 tests $95.00       
2206530 100 tests $190.00       
Clone: HECA-452
Isotype: Rat IgM, κ
Workshop Number: V S075
Reactivity: Human, Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation: The antibody was conjugated with FITC under optimal conditions, and is at >85% purity. The solution is free of unconjugated FITC.
Concentration: Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

The HECA-452 antibody cross-reacts with mouse skin homing lymphocytes4. Treatment of activated HUVEC cells with HECA-452 antibody inhibits lymphocyte adhesion. Additional reported applications (for the relevant formats) include: blocking of lymphocyte binding to E-selectin3, and immunohistochemistry1,2 of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Duijvestijn AM, et al. 1988. Am. J. Pathol. 130:147. (IHC)
2. Picker LJ, et al. 1991. Nature 349:796. (IHC)
3. Berg EL, et al. 1991. J. Exp. Med. 174:1461.
4. Borges E, et al. 1997. J. Biol. Chem. 272:28786.
5. Ren YL, et al. 2012. Am J Clin Pathol. 138:435. PubMed

Human peripheral blood lymphocytes stained

Human peripheral blood lymphocytes stained with HECA-452 FITC



Description:

Cutaneous lymphocyte antigen (CLA) is a 140 kD homodimer protein recognized by a unique mAb, HECA-452. It is expressed on T cells in skin, subsets of peripheral blood memory T cells, NK cells, memory B cells and dendritic cells as well as on monocytes, granulocytes, and activated endothelial cells. CLA is a carbohydrate epitope of sialic acid and fucose-modified P-selectin glycoprotein ligand-1 (PSGL-1), a surface glycoprotein expressed on the majority of peripheral blood leukocytes. CLA is a ligand for E-selectin, P-selectin, and L-selectin. It plays a role in memory lymphocyte homing, tethering, and rolling.

Other Names: Cutaneous Lymphocyte-associated Antigen (CLA)
Structure: 140 kD, carbohydrate epitope of PSGL-1
Distribution: T cells in skin, subsets of peripheral blood T cells, NK cells, B cells and dendritic cells, monocytes, granulocytes
Function: Memory cells tethering and rolling
Ligand Receptor: E-selectin, P-selectin, L-selectin
Antigen References:

1. Picker LJ, et al. 1990. Am. J. Pathol. 136:1053.
2. Berg EL, et al. 1991. J. Exp. Med. 174:1461.
3. Fuhlbrigge RC, et al. 1997. Nature 389:978.
4. Tu L, et al. 1999. J. Exp. Med. 189:241.
5. Yoshino T, et al. 1999. Cell. Immunol. 197:39.
6. Chang SE, et al. 2003. Acta Derm-Venereol. 83:162.
7. Schakel K, et al. 2002. Immunity 17:289.
8. Fuhlbrigge RC, et al. 2002. J. Immunol. 168:5645.