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Safety Data Sheet (SDS) PE Anti-mouse CD49b Antibody     Product Data Sheet (PDF)    
PE Anti-mouse CD49b Antibody
1117530 200 µg $255.00       
Clone: HMα2
Isotype: Armenian Hamster IgG
Reactivity: Mouse
Immunogen: Mouse colon carcinoma cell line Colon26
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunofluorescence on frozen sections4, and blocking of cell adhesion1-3.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Miyake S, et al. 1994. Eur. J. Immunol. 24:2000. (Block, IP)
2. Noto K, et al. 1995. Int. Immunol. 7:835. (Block)
3. Arase H, et al. 2001. J. Immunol. 167:1141. (Block)
4. Zhang Z, et al. 2008. Blood 111:1980. (IF)

C57BL/6 splenocytes stained with HMα2

C57BL/6 splenocytes stained with HMα2 PE and 145-2C11 FITC



Description:

CD49b is a 150 kD glycoprotein, also known as α2 integrin, VLA-2 α chain, Integrin α2 chain, and HMα2. It is a member of the integrin family, expressed on NK cells, a subset of splenic CD4+ T cells, NK-T cells, intestinal intraepithelial and lamina propria lymphocytes, epithelial cells, and platelets. By associating with CD29 (integrin β1 subunit), CD49b forms the VLA-2 (integrin α2β1 ) complex. It plays a critical role in both adhesion and lymphocyte activation. The primary ligands for CD49b are collagen, laminin, and fibronectin. The HMα2 antibody has been shown to be useful for partially blocking CD49b mediated interactions with collagen. Additionally, this antibody blocks staining of splenic NK cells by the monoclonal antibody DX5.

Other Names: α2 integrin, VLA-2 α chain, Integrin α2 chain, ITGA2, HMa2
Structure: Integrin family, associates with CD29 (β1 integrin), 150 kD
Distribution: NK cells, subset splenic CD4+ T cells, NK-T cells, intestinal intraepithelial and lamina propria lymphocytes, epithelial cells, platelets
Function: Adhesion, lymphocyte activation
Ligand Receptor: Collagen, laminin, fibronectin
Antigen References:

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Sasaki K, et al. 2003. Int. Immunol. 15:701.
3. Inoue O, et al. 2003. J. Cell Biol. 160:769.