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Safety Data Sheet (SDS) Biotin Anti-mouse/rat CD49e Antibody     Product Data Sheet (PDF)    
Biotin Anti-mouse/rat CD49e Antibody
1119520 500 µg $235.00       
Clone: HMα5-1
Isotype: Armenian Hamster IgG
Reactivity: Mouse, Rat
Immunogen: Affinity-purified mouse VLA-5 protein (α5β1, CD49e/CD29)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application:

FC - Quality tested
IP - Reported in the literature

Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vivo inhibition of fibronectin receptor (VLA-5) functions and in vitro blocking of CD49e mediated interactions 1,2.
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References:

1. Yasuda M, et al. 1995. Int. Immunol. 7:251. (IP, Block)
2. Noto K, et al. 1995. Int. Immunol. 7:835. (Block)
3. Lin JS, et al. 2010. J. Leukoc. Biol. 88:95. PubMed

C57BL/6 mouse bone marrow cells

C57BL/6 mouse bone marrow cells were stained with CD45R/B220 APC and biotinylated CD49e (clone HMα5-1) (top), or biotinylated Armenian hamster IgG isotype control (bottom), followed by Sav-PE.





Description: CD49e is a 135 kD protein, also known as α5 integrin or VLA-5 α chain. It is a member of the integrin family, expressed on thymocytes, splenic B cells, activated T cells, and mast cells. CD49e associates with CD29 (integrin β1 chain) to form the fibronectin receptor (VLA-5). CD49e plays a critical role in both adhesion and T cell costimulation. The primary ligand for CD49e/CD29 (VLA-5) is fibronectin. The HMα5-1 antibody has been shown to partially block CD49e mediated interactions. It has also been observed that plate-bound HMα5-1 antibody enhances IgE mediated degranulation and that in vivo injection of HMα5-1 antibody, in combination with anti-CD49d and anti-CD61 antibodies, blocks passive cutaneous anaphylaxis.
Other Names: α5 integrin, VLA-5 α chain, Integrin α5 chain, ITGA5
Structure: Integrin family, associates with integrin β1, 135 kD
Distribution: Activated T cells, mast cells
Function: Adhesion, costimulation
Ligand Receptor: Fibronectin
Antigen References:

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Kinashi T, et al. 1994 Blood Cells 20:25.
3. Hemler ME. 1990. Annu. Rev. Immunol. 8:365.