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Safety Data Sheet (SDS) PE/Cy7 Anti-mouse CD86 Antibody     Product Data Sheet (PDF)    
PE/Cy7 Anti-mouse CD86 Antibody
1125575 25 µg $85.00       
1125580 100 µg $235.00       
Clone: PO3
Isotype: Rat IgG2b, κ
Reactivity: Mouse
Immunogen: BALB/c mouse B leukemia cell line BCL1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE/Cy7 under optimal conditions. The solution is free of unconjugated PE/Cy7 and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: in vivo and in vitro blocking 1,4,5 of autoantibody production and T cell activation, stimulation of B cell activity3, immunoprecipitation2, and immunohistochemical staining2 of acetone-fixed frozen sections.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Nakajima A, et al. 1995. Eur. J. Immunol. 25:3060.
2. Nuriya S, et al. 1996. Int. Immunol. 8:917.
3. Kasprowicz DJ, et al. 2000. J. Immunol. 165:680.
4. Saito K, et al. 1998. J. Immunol. 160:4225.
5. Nakajima A, et al. 1998. J. Immunol. 161:1901.
6. Ma XT, et al. 2006. Cancer Research 66:1169.
7. Lawson BR, et al. 2007. J. Immunol. 178:5366.
8. Bhatnagar S and Schorey JS. 2007. J. Biol. Chem. doi:10.1074/jbc.M702277200.
9. Giroux M, et al. 2007. J. Immunol. 179:4492.

C57BL/6 mouse splenocytes stained with

C57BL/6 mouse splenocytes stained with PO3 PE/Cy7



Description:

CD86 is an 80 kD immunoglobulin superfamily member, also known as B7-2, B70, and Ly-58. CD86 is expressed on activated B and T cells, macrophages, dendritic cells and astrocytes. CD86 along with CD80 are the ligands of CD28 and CD152 (CTLA-4). CD86 is expressed earlier in the immune response than CD80. CD86 has also been shown to be involved in immunoglobulin class-switching and triggering of NK cell-mediated cytotoxicity. CD86 binds to CD28 to transduce co-stimulatory signals for T cell activation, proliferation, and cytokine production. CD86 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells. The PO3 antibody has been shown to block autoantibody production in vivo and inhibit T cell activation in vitro.

Other Names: B7-2, B70, Ly-58
Structure: Ig superfamily, 80 kD
Distribution: B cells and T cells (upregulated upon activation), macrophages, dendritic cells, and astrocytes
Function: T cell costimulation, Ig class-switching, NK cell cytotoxicity
Ligand Receptor: CD28, CD152 (CTLA-4)
Antigen References:

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Hathcock KS, et al. 1993. Science 262:905.
3. Freeman GJ, et al. 1993. Science 262:907.
4. Carreno BM, et al. 2002. Annu. Rev. Immunol. 20:29.


This product or portions thereof is manufactured under license from GE Healthcare under U.S. Patent Numbers 5,268,486; 5,569,587; 5,627,027 and patents or pending applications that are continuations, continuations-in-part, re-examinations, divisionals, reissues or foreign equivalents thereof. THIS MATERIAL IS SUBJECT TO PROPRIETARY RIGHTS OF GE HEALTHCARE BIO-SCIENCES CORP. AND CARNEGIE MELLON UNIVERSITY AND MADE AND SOLD UNDER LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. THIS PRODUCT IS LICENSED FOR SALE ONLY FOR RESEARCH. IT IS NOT LICENSED FOR ANY OTHER USE. THERE IS NO IMPLIED LICENSE HEREUNDER FOR ANY COMMERCIAL USE.
COMMERCIAL USE shall include:

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