The antibody was purified by affinity chromatography.
Storage & Handling:
The antibody solution should be stored undiluted between 2°C and 8°C.
FC - Quality tested
Additional reported applications (for the relevant formats) include: To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 , is recommended prior to immunofluorescent staining.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
1. Yamashita Y, et al. 1999. J. Immunol. 162:5940. 2. Kouro T, et al. 2002. Blood 100:3672. 3. Maeda K, et al. 2005. Blood 106:879. 4. Diao J, et al. 2004. J. Immunol. 173:1826.
C57BL/6 mouse splenocytes stained with purified SB/199, followed by biotinylated anti-rat IgG and Sav-PE
CD127 is a 60-90 kD type I transmembrane glycoprotein, also known as IL-7 receptor α chain or IL-7Rα. It forms heterodimer with the common γ chain (γc or CD132) which is shared with the receptors for IL-2, IL-4, IL-9, IL-13, IL-15, and IL-21. CD127 is expressed on immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, and bone marrow stromal cells. CD127 has been reported to be an useful marker for identifying memory and effector T cells. The ligation of IL-7 with its receptor is important for stimulation of mature and immature T cells as well as immature B cells proliferation and development.
IL-7 receptor α chain, IL-7Rα
Type I transmembrane glycoprotein, associate with CD132, 60-90 kD
Immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, bone marrow stromal cells
T cell and immature B cell proliferation and development
1. Sudo T, et al. 1993. Proc. Natl. Acad. Sci. USA. 90:9125. 2. He YW and Malek TR. 1998. Crit Rev. Immunol. 18:503. 3. Huster K M,et al. 2004. Proc. Natl. Acad. Sci. USA. 101:5610. 4. Pillai M, et al. 2004. Leuk Lymphoma. 45:2403. 5. Morrissey PJ, et al. 1989. J. Exp. Med. 169:707.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Sony, the Sony logo, Sony Biotechnology Inc. and logo, iCyt, the iCyt logo EC800 and SY3200 are trademarks of Sony Corporation. Alexa Fluor® and Pacific Blue™ are registered trademarks of and licensed under patents assigned to Life Technologies Corporation. Cy and CyDye are trademarks of GE Healthcare Limited. iCyt products containing Cy5, Cy5.5 or Cy7 are manufactured under license from GE Healthcare under U.S. Patent Numbers 5,569,587; 5,627,027 and patents or pending applications that are continuations, continuations-in-part, re-examinations, divisionals, reissues or foreign equivalents thereof. Brilliant Violet™, Brilliant Violet 421™, BV421™, Brilliant Violet 570™, BV570™, Brilliant Violet 605™, BV605™, Brilliant Violet 650™, and BV650™, Brilliant Violet 510™, BV510™, Brilliant Violet 711™, BV711™, Brilliant Violet 785™ and BV785™ are trademarks of Sirigen Group Ltd. PE-Dazzle 594™ is a trademark of Biolegend. All other trademarks are property of their respective owners. For Research Use Only. Not for use in diagnostic or therapeutic procedures.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.