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Safety Data Sheet (SDS) FITC Anti-mouse CD127 IL-7Ralpha Antibody     Product Data Sheet (PDF)    
FITC Anti-mouse CD127 (IL-7Rα) Antibody
1205525 50 µg $95.00       
1205530 500 µg $345.00       
Clone: SB/199
Isotype: Rat IgG2b, κ
Reactivity: Mouse
Immunogen: mouse pre-B cell line 1A9
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 , is recommended prior to immunofluorescent staining.

Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References: 1. Yamashita Y, et al. 1999. J. Immunol. 162:5940.
2. Kouro T, et al. 2002. Blood 100:3672.
3. Maeda K, et al. 2005. Blood 106:879.
4. Diao J, et al. 2004. J. Immunol. 173:1826.
C57BL/6 mouse splenocytes stained with

C57BL/6 mouse splenocytes stained with CD3 (145-2C11 APC) and SB/199 FITC

Description: CD127 is a 60-90 kD type I transmembrane glycoprotein, also known as IL-7 receptor α chain or IL-7Rα. It forms heterodimer with the common γ chain (γc or CD132) which is shared with the receptors for IL-2, IL-4, IL-9, IL-13, IL-15, and IL-21. CD127 is expressed on immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, and bone marrow stromal cells. CD127 has been reported to be an useful marker for identifying memory and effector T cells. The ligation of IL-7 with its receptor is important for stimulation of mature and immature T cells as well as immature B cells proliferation and development.
Other Names: IL-7 receptor α chain, IL-7Rα
Structure: Type I transmembrane glycoprotein, associate with CD132, 60-90 kD
Distribution: Immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, bone marrow stromal cells
Function: T cell and immature B cell proliferation and development
Ligand Receptor: IL-7
Antigen References: 1. Sudo T, et al. 1993. Proc. Natl. Acad. Sci. USA. 90:9125.
2. He YW and Malek TR. 1998. Crit Rev. Immunol. 18:503.
3. Huster K M,et al. 2004. Proc. Natl. Acad. Sci. USA. 101:5610.
4. Pillai M, et al. 2004. Leuk Lymphoma. 45:2403.
5. Morrissey PJ, et al. 1989. J. Exp. Med. 169:707.