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Safety Data Sheet (SDS) Alexa Fluor 488 Anti-mouse CD127 IL-7Ralpha Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 488 Anti-mouse CD127 (IL-7Rα) Antibody
1205535 25 µg $90.00       
Clone: SB/199
Isotype: Rat IgG2b, κ
Reactivity: Mouse
Immunogen: mouse pre-B cell line 1A9
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 , is recommended prior to immunofluorescent staining.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Application References: 1. Yamashita Y, et al. 1999. J. Immunol. 162:5940.
2. Kouro T, et al. 2002. Blood 100:3672.
3. Maeda K, et al. 2005. Blood 106:879.
4. Diao J, et al. 2004. J. Immunol. 173:1826.
C57BL/6 mouse splenocytes stained with

C57BL/6 mouse splenocytes stained with CD3 APC and SB/199 Alexa Fluor® 488



Description: CD127 is a 60-90 kD type I transmembrane glycoprotein, also known as IL-7 receptor α chain or IL-7Rα. It forms heterodimer with the common γ chain (γc or CD132) which is shared with the receptors for IL-2, IL-4, IL-9, IL-13, IL-15, and IL-21. CD127 is expressed on immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, and bone marrow stromal cells. CD127 has been reported to be an useful marker for identifying memory and effector T cells. The ligation of IL-7 with its receptor is important for stimulation of mature and immature T cells as well as immature B cells proliferation and development.
Other Names: IL-7 receptor α chain, IL-7Rα
Structure: Type I transmembrane glycoprotein, associate with CD132, 60-90 kD
Distribution: Immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, bone marrow stromal cells
Function: T cell and immature B cell proliferation and development
Ligand Receptor: IL-7
Antigen References: 1. Sudo T, et al. 1993. Proc. Natl. Acad. Sci. USA. 90:9125.
2. He YW and Malek TR. 1998. Crit Rev. Immunol. 18:503.
3. Huster K M,et al. 2004. Proc. Natl. Acad. Sci. USA. 101:5610.
4. Pillai M, et al. 2004. Leuk Lymphoma. 45:2403.
5. Morrissey PJ, et al. 1989. J. Exp. Med. 169:707.

This product is provided under an agreement between Life Technologies Corporation and Sony Biotechnology Inc. and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications, and corresponding non-U.S. equivalents, owned by and/or licensed to Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research and/or development conducted by the buyer (whether the buyer is an academic or for profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to (1) use of the product or its components in manufacturing, (2) use of the product or its components for therapeutic, diagnostic or prophylactic purposes, or (3) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Molecular Probes Detection Technologies, Attn: Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.