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Safety Data Sheet (SDS) Alexa Fluor 488 Anti-mouse Panendothelial Cell Antigen Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 488 Anti-mouse Panendothelial Cell Antigen Antibody
1202525 25 µg $90.00       
1202530 100 µg $190.00       
Clone: MECA-32
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: Mouse lymph node stromal cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (in the relevant formats) include: immunoprecipitation, Western blotting, immunocytochemistry, and immunohistochemical staining of acetone-fixed frozen sections2-5. Clone MECA-32 is not recommended for formalin-fixed paraffin sections.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Application References:

1. Leppink DM, et al. 1989. Transplantation 48:874.
2. Bergese SD, et al. 1994. Transplantation 57:711. (IHC)
3. Tanneau GM, et al. 1999. J. Histochem. Cytochem. 47:1581. (IHC)
4. Kopp HG, et al. 2005. Blood 106:505. (IHC)
5. Hallmann R, et al. 1995. Dev. Dyn. 202:325. (IHC)

bEND.3 cells stained with MECA-32

bEND.3 cells stained with MECA-32 Alexa Fluor® 488



Description:

MECA-32 is a 50-55 kD homodimer glycoprotein primarily expressed on most endothelial cells in the embryonic and the adult mouse tissues, except in the brain and in skeletal and cardiac muscle, where it has more restricted distribution. In skeletal and cardiac muscle, the MECA-32 antigen is confined in small arterioles and venules. But in some conditions, such as inflammation or cardiac transplantation, MECA-32 expression can be induced on some negative-cardiac endothelial cells. In the brain, its expression occurs only in the circumventricular organs and the neurohemal tissues. During embryonic development, MECA-32 antigen is expressed on the brain vasculature up to day 16 of gestation, and disappears at day 17 and after.

Other Names: Pan-endothelial Cell Antigen, MECA-32, Plasmalemma vesicle-associated protein
Structure: 50-55 kD homodimer
Distribution: Endothelial cell in the embryonic and the adult mice, except in the brain and in skeletal and cardiac muscle, where it has more restricted distribution
Antigen References:

1. Leppnik DM, et al. 1989. Transplantation 48:874.
2. Hallmann R, et al. 1995. Dev. Dyn. 202:325.
3. Kruse A, et al. 1999. Bio. Reprod. 61:1393.


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