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Safety Data Sheet (SDS) Alexa Fluor 488 Anti-mouse MAdCAM-1 Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 488 Anti-mouse MAdCAM-1 Antibody
1203535 25 µg $90.00       
1203540 100 µg $190.00       
Clone: MECA-367
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: Endothelial cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: in vitro and in vivo blocking of lymphocyte adhesion and in vivo blocking of lymphocyte homing1-4,7, immunohistochemical staining1,5,6 of acetone-fixed frozen sections, immunoprecipitation, and Western blotting1

.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Application References:

1. Streeter PR, et al. 1988. Nature 331:41.
2. Briskin MJ, et al. 1993. Nature 363:461.
3. Berlin C, et al. 1993. Cell 74:185.
4. Bargatze RF, et al. 1995. Immunity 3:99.
5. Tanneau GM, et al. 1999. J. Histochem. Cytochem. 47:1581.
6. Savinov AY, et al. 2003. J. Exp. Med. 197:643.
7. Rivera-Nieves J, et al. 2005. J. Immunol. 174:2343.
8. Kometani K, et al. 2011. J. Exp Med. 208:1447. PubMed.
9. Morrison BJ,< et al. 2012. PLoS One. 7:e52692. PubMed.

TNFα-stimulated bEND.3 cells were stained

TNFα-stimulated bEND.3 cells were stained with anti-mouse MAdCAM-1 (clone MECA-367) Alexa Fluor® 488 (filled histogram) or mouse IgG2a, κ Alexa Fluor® 488 isotype control (open histogram).



Description:

MAdCAM-1 is a 58-66kD type I glycoprotein, also known as Mucosal addressin cell adhesion molecule-1. This mucosal vascular addressin is a member of the Ig superfamily found on fetus and neonatal endothelial cells. In adults, MAdCAM-1 is predominately expressed on high endothelial venule (HEV) of Peyer's patches, mesenteric lymph nodes and gut lamina propria. It is also expressed on vascular endothelium in mammary glands and pancreas. MAdCAM-1, through its interaction with integrin α4β7 or CD62L, is involved in lymphocyte tethering, rolling, and homing. It has been reported that immobilized MAdCAM-1 is able to co-stimulate T cell proliferation. The MECA-367 antibody blocks the interaction of MAdCAM-1 with its counter-receptor both in vitro and in vivo. In vivo administration of the mAb is able to reduce T-cell mediated inflammation in some gastrointestinal diseases.

Other Names: Mucosal addressin cell adhesion molecule-1
Structure: Type I glycoprotein, Ig superfamily
Distribution: HEV of mucosal lymphoid organ and lamina propia, endothelial cells in mammary glands and pancreas
Function: Lymphocyte tethering, rolling and homing, costimulate T cell proliferatioon
Ligand Receptor: Integrin a4ß7, CD62L
Antigen References:

1. Streeter PR, et al. 1988. Nature 331:41
2. Briskin MJ, et al. 1993.Nature 363:461.
3. Berlin C, et al. 1993. Cell 74:185.
4. Lehnert K, et al. 1998. Eur. J. Immunol. 28:3605.
5. Picarella D, et al. 1997. J. Immunol. 158:2099.


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