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Safety Data Sheet (SDS) Alexa Fluor 488 Anti-mouse MAdCAM-1 Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 488 Anti-mouse MAdCAM-1 Antibody
1203535 25 µg $90.00       
1203540 100 µg $190.00       
Clone: MECA-367
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: Endothelial cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: in vitro and in vivo blocking of lymphocyte adhesion and in vivo blocking of lymphocyte homing1-4,7, immunohistochemical staining1,5,6 of acetone-fixed frozen sections, immunoprecipitation, and Western blotting1


Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Application References:

1. Streeter PR, et al. 1988. Nature 331:41.
2. Briskin MJ, et al. 1993. Nature 363:461.
3. Berlin C, et al. 1993. Cell 74:185.
4. Bargatze RF, et al. 1995. Immunity 3:99.
5. Tanneau GM, et al. 1999. J. Histochem. Cytochem. 47:1581.
6. Savinov AY, et al. 2003. J. Exp. Med. 197:643.
7. Rivera-Nieves J, et al. 2005. J. Immunol. 174:2343.
8. Kometani K, et al. 2011. J. Exp Med. 208:1447. PubMed.
9. Morrison BJ,< et al. 2012. PLoS One. 7:e52692. PubMed.

TNFα-stimulated bEND.3 cells were stained

TNFα-stimulated bEND.3 cells were stained with anti-mouse MAdCAM-1 (clone MECA-367) Alexa Fluor® 488 (filled histogram) or mouse IgG2a, κ Alexa Fluor® 488 isotype control (open histogram).


MAdCAM-1 is a 58-66kD type I glycoprotein, also known as Mucosal addressin cell adhesion molecule-1. This mucosal vascular addressin is a member of the Ig superfamily found on fetus and neonatal endothelial cells. In adults, MAdCAM-1 is predominately expressed on high endothelial venule (HEV) of Peyer's patches, mesenteric lymph nodes and gut lamina propria. It is also expressed on vascular endothelium in mammary glands and pancreas. MAdCAM-1, through its interaction with integrin α4β7 or CD62L, is involved in lymphocyte tethering, rolling, and homing. It has been reported that immobilized MAdCAM-1 is able to co-stimulate T cell proliferation. The MECA-367 antibody blocks the interaction of MAdCAM-1 with its counter-receptor both in vitro and in vivo. In vivo administration of the mAb is able to reduce T-cell mediated inflammation in some gastrointestinal diseases.

Other Names: Mucosal addressin cell adhesion molecule-1
Structure: Type I glycoprotein, Ig superfamily
Distribution: HEV of mucosal lymphoid organ and lamina propia, endothelial cells in mammary glands and pancreas
Function: Lymphocyte tethering, rolling and homing, costimulate T cell proliferatioon
Ligand Receptor: Integrin a4ß7, CD62L
Antigen References:

1. Streeter PR, et al. 1988. Nature 331:41
2. Briskin MJ, et al. 1993.Nature 363:461.
3. Berlin C, et al. 1993. Cell 74:185.
4. Lehnert K, et al. 1998. Eur. J. Immunol. 28:3605.
5. Picarella D, et al. 1997. J. Immunol. 158:2099.

This product is provided under an agreement between Life Technologies Corporation and Sony Biotechnology Inc. and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications, and corresponding non-U.S. equivalents, owned by and/or licensed to Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research and/or development conducted by the buyer (whether the buyer is an academic or for profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to (1) use of the product or its components in manufacturing, (2) use of the product or its components for therapeutic, diagnostic or prophylactic purposes, or (3) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Molecular Probes Detection Technologies, Attn: Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.