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Safety Data Sheet (SDS) PE Anti-mouse CD254 TRANCE RANKL Antibody     Product Data Sheet (PDF)    
PE Anti-mouse CD254 (TRANCE, RANKL) Antibody
3150025 50 µg $155.00       
3150030 200 µg $325.00       
Clone: IK22/5
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: NSO-derived recombinant mouse TRANCE
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

The IK22/5 antibody has been reported to block the binding of RANK ligand-Fc to RANK. Additional reported applications (for the relevant formats) include: immunoprecipitation2, Western blotting2, and blocking of ligand binding1,2,4.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric analysis, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Miyahira Y, et al. 2003. J. Immunol. 171:6344. (Block)
2. Personal communication (Block IP WB)
3. Gao Y, et al. 2007. J. Clin. Invest. 117:122.
4. Kamijo S, et al. 2006. Biochem. Biophys. Res. Commun. 347:124. (Block)
5. Fionda C, et al. 2007. J. Immunol. 178:4039.
6. Haslam SZ, et al. 2008. Endocrinology. 149:2098. (Block) PubMed
7. Takeshita S, et al. 2014. J Biol Chem. 289:16699. PubMed

TRANCE transfected cells stained with

TRANCE transfected cells stained with IK22-5 PE



Description:

CD254 is a 19 kD TNF superfamily member also known as TRANCE (TNF-related activation induced cytokine), RANK ligand, RANKL, TNFSF11, OPGL, and ODF. TRANCE is expressed on activated T cells and osteoclasts and has been implicated in the regulation of T cell and dendritic cell interactions as well as osteoclast differentiation.

Other Names: Tumor necrosis factor-related activation-induced cytokine, Receptor activator of NF-κB ligand (RANK L), Osteoprotegerin ligand (OPGL), Osteoclast differentiation factor (ODF), SOFA, TNFSF-11, CD254, RANKL
Structure: TNF superfamily; 19 kD (Mammalian)
Distribution: Activated T cells, osteoclasts
Function: Regulates T and dendritic cell interactions, and osteoclast differentiation
Ligand Receptor: RANK, OPG
Regulation: Type II integral membrane protein processed by TACE for secretion; upregulated upon TCR engagement
Cellular Sources: Activated T cells, myeloid bone marrow precursors, stromal fibroblasts, synovial cells, osteoblasts
Cellular Targets: Dendritic cells, osteoclasts
Receptors: ODFR, ODAR, TRANCE-R
Bioactivity/Activities: Activates JNK and NF-κB; involved in bone resorption; induces the expression of IL-1, IL-6, IL-12, IL-15 in dendritic cells
Antigen References:

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Josien R, et al. 1999. J. Immunol. 162:2562.
3. Takayanagi H, et al. 2000. Nature 408:600.
4. Wong B, et al. 1999. Mol. Cell. 4:1041.