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Safety Data Sheet (SDS) Alexa Fluor 488 Anti-mouse CD31 Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 488 Anti-mouse CD31 Antibody
1112065 25 µg $90.00       
1112070 100 µg $190.00       
Clone: 390
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: C3H/HeJ mouse hematopoietic progenitor cell line 3
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested
IHC , IF - Reported in the literature

Application Notes:

Anti-mouse CD31 clones 390 and MEC13.3 bind to their respective non-overlapping epitopes in IgD2 of CD31.8 Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro and in vivo blocking of CD31-mediated cell-cell interactions1-4, and immunohistochemical staining5,6,7 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections. Special Note: This antibody is not recommended for formalin-fixed paraffin-embedded sections.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Application References:

1. Baldwin HS, et al. 1994. Development 120:2539. (IP, Block)
2. DeLisser HM, et al. 1997. Am. J. Pathol. 151:671. (Block)
3. Rosenblum WI, et al. 1996. Stroke 27:709. (Block)
4. Iguchi A, et al. 1997. Cell Struct. Funct. 22:357. (Block)
5. Wyder L, et al. 2000. Cancer Res. 60:4682. (IHC)
6. Wiewrodt R, et al. 2002. Blood 99:912. (IHC)
7. McQualter JL, et al. 2009. Stem Cells. 27:623. (IHC) PubMed
8. Chacko AM, et al. 2012. PLoS One 7:e34958.
9. Hsieh CY, et al. 2014. J Immunol. 193:3693. PubMed
10. Watanabe S, et al. 2015. J Virol. 89:5847. PubMed

C57BL/6 mouse splenocytes stained with

C57BL/6 mouse splenocytes stained with 390 Alexa Fluor® 488



Description:

CD31 is a 130-140 kD glycoprotein, also known as platelet endothelial cell adhesion molecule (PECAM-1) and EndoCAM. It is a member of the Ig superfamily, expressed on endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, and T and B cell subsets, and is critical for cell-cell interactions. The primary ligands for CD31 have been reported to be CD38 and the vitronectin receptor (αv β3 integrin, CD51/CD61). Other reported functions of CD31 are neutrophil emigration to sites of inflammation and angiogenesis.

Other Names: PECAM-1, EndoCAM
Structure: Ig superfamily, 130-140 kD
Distribution: Endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, T and B cell subsets
Function: Adhesion
Ligand Receptor: CD38, αV3 integrin
Antigen References:

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. DeLisser HM, et al. 1994. Immunol. Today 15:490.
3. Newman PJ, et al. 1990. Science 247:1219.


This product is provided under an agreement between Life Technologies Corporation and Sony Biotechnology Inc. and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications, and corresponding non-U.S. equivalents, owned by and/or licensed to Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research and/or development conducted by the buyer (whether the buyer is an academic or for profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to (1) use of the product or its components in manufacturing, (2) use of the product or its components for therapeutic, diagnostic or prophylactic purposes, or (3) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Molecular Probes Detection Technologies, Attn: Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.