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Safety Data Sheet (SDS) Alexa Fluor 488 Anti-mouse CD40 Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 488 Anti-mouse CD40 Antibody
1114550 100 µg $190.00       
Clone: HM40-3
Isotype: Armenian Hamster IgM
Reactivity: Mouse
Immunogen: (BALB/c x NZB)F1 mouse-derived lymphoma WEHI 231
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was conjugated with Alexa Fluor® 488 under optimal conditions, and is at >85% purity.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested
IHC, IF - Reported in the literature

Application Notes:

Additional reported applications (for the relevant formats) include: blocking of CD40 ligand binding1, stimulation of mouse B cell and dendritic cell proliferation2-6,9, and immunohistochemistry7 of acetone-fixed frozen sections.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Application References:

1. Kawano T, et al. 1997. Science 278:1626. (Block)
2. Kaneko Y, et al. 1996. Eur. J. Immunol. 26:3061. (Costim)
3. Kashiwada M, et al. 1996. Eur. J. Immunol. 26:1451. (Costim)
4. Inaba M, et al. 1995. Eur. J. Immunol. 25:1244. (Costim)
5. Akiba H, et al. 1999. J. Immunol. 162:7058. (Costim)
6. Ridge JP, et al. 1998. Nature 393:474. (Costim)
7. Morelli AE, et al. 2000. Transplantation 69:2647. (IHC)
8. Ma XT, et al. 2006. Cancer Research 66:1169.
9. Yu X, et al. 2006. J. Biol. Chem. 281:15505. (Costim)
10. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed

C57BL/6 mouse splenocytes stained with

C57BL/6 mouse splenocytes stained with HM40-3 Alexa Fluor® 488



Description:

CD40 is a 48 kD type I transmembrane glycoprotein, also known as Bp50. It is a member of the tumor necrosis factor receptor (TNFR) superfamily, expressed on B cells, basal epithelial cells, macrophages, follicular dendritic cells, endothelial cells, and a subset of CD34+ hematopoietic progenitors. CD40 regulates B cell development/maturation, Ig isotype switching and, in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with its ligand CD154 (gp39) expressed on activated T cells, is important in costimulation and immune regulation. The HM40-3 antibody has been reported to stimulate dendritic and B cell proliferation and block CD40-CD40L interactions.

Other Names: Bp50
Structure: TNFR superfamily, 48 kD
Distribution: B cells, basal epithelial cells, macrophages, follicular dendritic cells, endothelial cells, CD34+ hematopoietic progenitors
Function: Stimulates B cell growth, differentiation and isotype switching, stimulates macrophage and dendritic cell cytokine production
Ligand Receptor: CD40L (CD154)
Antigen References:

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Bancherou J, et al. 1994. Annu. Rev. Immunol. 12:881.
3. Clark EA, et al. 1996. P. Natl. Acad. Sci. USA 83:4494.


This product is provided under an agreement between Life Technologies Corporation and Sony Biotechnology Inc. and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications, and corresponding non-U.S. equivalents, owned by and/or licensed to Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research and/or development conducted by the buyer (whether the buyer is an academic or for profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to (1) use of the product or its components in manufacturing, (2) use of the product or its components for therapeutic, diagnostic or prophylactic purposes, or (3) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Molecular Probes Detection Technologies, Attn: Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.