The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™ .
Storage & Handling:
The CD44 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
Clone IM7 has been reported to recognize an epitope common to alloantigens and all isoforms of CD4417,18 that is located between amino acids 145 and 18620. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections6,7, complement-mediated cytotoxicity1, immunoprecipitation1,3, and in vivo inhibition of DTH4,5.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
1. Trowbridge IS, et al. 1982. Immunogenetics 15:299. (ICFC, IP, CMCD) 2. Katoh S, et al. 1994. J. Immunol. 153:3440. (ELISA) 3. Budd RC, et al. 1987. J. Immunol. 138:3120. (IP) 4. Camp RL, et al. 1993. J. Exp. Med. 178:497. (Block) 5. Weiss JM, et al. 1997. J. Cell Biol. 137:1137. (Block) 6. Frank NY, et al. 2005. Cancer Res. 65:4320. (IHC) PubMed 7. Cuff CA, et al. 2001. J. Clin. Invest. 108:1031. (IHC) 8. Lee JW, et al. 2006. Nature Immunol. 8:181. 9. Zhang N, et al. 2005. J. Immunol. 174:6967. PubMed 10. Huabiao C, et al. 2005. J. Immunol. 175:591. PubMed 11. Gui J, et al. 2007. Int. Immunol. 19:1201. PubMed 12. Wang XY, et al. 2008. Blood 111:2436. PubMed 13. Kenna TJ, et al. 2008. Blood 111:2091. PubMed 14. Yamazaki J, et al. 2009. Blood PubMed 15. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed 16. Chen YW, et al. 2010. Mol. Cancer Ther. 9:2879. PubMed 17. Zheng Z, et al. 1995. J. Cell. Biol. 130:485. 18. Wiranowska M, et al. 2010. Int. J. Cancer 127:532. 19. Hirokawa Y, et al. 2014. Am J Physiol Gastrointerest Liver Physiol. 306:547. PubMed 20. Sandmaier BM, et al. 1998. Blood 91:3494.
C57BL/6 Mouse Splenocytes stained with IM7 Pacific Blue™
CD44 is a 80-95 kD glycoprotein also known as Hermes, Pgp1, H-CAM, or HUTCH. It is expressed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells. As B and T cells become activated or progress to the memory stage, CD44 expression increases from low or mid levels to high levels. Thus, CD44 has been reported to be a valuable marker for memory cell subsets. High CD44 expression on Treg cells has been associated with potent suppressive function via high production of IL-10. CD44 is an adhesion molecule involved in leukocyte attachment to and rolling on endothelial cells, homing to peripheral lymphoid organs and to the sites of inflammation, and leukocyte aggregation.
Hermes, Pgp-1, H-CAM, HUTCH-1, ECMR III, gp85, Ly-24
Variable splicing of CD44 gene generates many CD44 isoforms, 80-95 kD
All leukocytes, epithelial cells, endothelial cells, hepatocytes, mesenchymal cells
Leukocyte attachment and rolling on endothelial cells, stromal cells and ECM
Hyaluronan, MIP-1β, fibronectin, collagen
1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Haynes BF, et al. 1991. Cancer Cells 3:347. 3. Goldstein LA, et al. 1989. Cell 56:1063. 4. Mikecz K, et al. 1995. Nat. Med. 1:558. 5. Hegde V, et al. 2008. J. Leukocyte Biol. 84:134. 6. Liu T, et al. 2009. Biol. Direct 4:40.
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