Sony Biotechnology Inc. Sony Biotechnology Inc.cart
About Us Products What's New Library News Calendar Contact Us Place Order
Safety Data Sheet (SDS) Pacific Blue Anti-mouse CD62L Antibody     Product Data Sheet (PDF)    
Pacific Blue™ Anti-mouse CD62L Antibody
1122115 25 µg $90.00       
1122120 100 µg $190.00       
Clone: MEL-14
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: C3H/eb mouse B lymphoma 38C-13
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: immunoprecipitation1-3, complement-dependent cytotoxicity4, in vivo and in vitro blocking of adhesion1-3,5, and immunohistochemical staining of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections6.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

Application References:

1. Gallatin WM, et al. 1983. Nature 304:30. (IP, Block)
2. Siegelman MH, et al. 1990. Cell 61:611. (IP, Block)
3. Lewinsohn DM, et al. 1987. J. Immunol. 138:4313. (IP, Block)
4. Iwabuchi K, et al. 1991. Immunobiology 182:161. (CMCD)
5. Pizcueta P, et al. 1994. Am. J. Pathol. 145:461.
6. Reichert RA, et al. 1986. J. Immunol. 136:3535. (IHC, FC)
7. Olver S, et al. 2006. Cancer Res. 66:571.
8. Fukushima A, et al. 2006. Invest. Ophthalmol. Vis. Sci. 47:657. PubMed
9. Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719. (FC) PubMed
10. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed
11. Lee JW, et al. 2006. Nature Immunol. 8:181.
12. Shigeta A, et al. 2008. Blood 112:4915 (FC) PubMed
13. de Vries VC, et al. 2009. Am. J. Transplant. 9:2270 PubMed

C57BL/6 mouse splenocytes were stained

C57BL/6 mouse splenocytes were stained with CD3 Alexa Fluor® 488 and CD62L (clone MEL-14) Pacific Blue™ (top) or rat IgG2a Pacific Blue™ isotype control (bottom).





Description:

CD62L is a 74-95 kD glycoprotein also known as L-selectin, LECAM-1, Ly-22, LAM-1, and MEL-14. It is a member of the selectin family and is expressed on the majority of B and naïve T cells, a subset of memory T cells, monocytes, granulocytes, most thymocytes, and a subset of NK cells. CD62L is important in lymphocyte homing to high endothelial venules (HEV) in peripheral lymph nodes and leukocyte "rolling" on activated endothelium. CD62L also contributes to neutrophil emigration at inflammatory sites. CD62L is rapidly shed from lymphocytes and neutrophils upon cellular activation and the expression levels of CD62L (in conjunction with other markers) have been used to distinguish naïve, effector, and memory T cells. CD62L has been reported to interact with CD34, GlyCAM-1, and MAdCAM-1.

Other Names: L-selectin, LECAM-1, Ly-22, LAM-1, MEL-14
Structure: Selectin, 95 kD (neutrophils) or 74 kD (lymphocytes)
Distribution: Subsets of B and T cells, monocytes, granulocytes, subset of NK cells
Function: Lymphocyte homing to HEV, rolling on activated endothelium
Ligand Receptor: CD34, GlyCAM-1, MAdCAM-1
Antigen References:

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Kishimoto TK, et al. 1990. P. Natl. Acad. Sci. USA 87:2244.
3. Tedder TF, et al. 1995. J. Exp. Med. 181:2259.


This product is provided under an agreement between Life Technologies Corporation and Sony Biotechnology Inc. and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications, and corresponding non-U.S. equivalents, owned by and/or licensed to Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research and/or development conducted by the buyer (whether the buyer is an academic or for profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to (1) use of the product or its components in manufacturing, (2) use of the product or its components for therapeutic, diagnostic or prophylactic purposes, or (3) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Molecular Probes Detection Technologies, Attn: Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.