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Safety Data Sheet (SDS) Alexa Fluor 647 Anti-mouse CD86 Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 647 Anti-mouse CD86 Antibody
1125095 25 µg $90.00       
1125100 100 µg $190.00       
Clone: GL-1
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: LPS-activated CBA/Ca mouse splenic B cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested
IHC, IF - Reported in the literature

Application Notes:

The GL-1 antibody can block the mixed lymphocyte reaction in vitro and has been shown to inhibit the priming of cytotoxic T lymphocytes in vivo (along with antibodies against B7-1). Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemical staining of acetone-fixed frozen sections2,6, immunofluorescence microscopy, and in vivo and in vitro blocking of T cell responses1-6. GL-1 is not suitable for immunohistochemical staining of formalin-fixed paraffin sections.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for other applications.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.

Application References:

1. Hathcock KS, et al. 1993. Science 262:905. (Block, IP)
2. Inaba KM, et al. 1994. J. Exp. Med. 180:1849. (Block, IHC)
3. Hathcock KS, et al. 1994. J. Exp. Med. 180:631. (Block)
4. Krummel MF, et al. 1995. J. Exp. Med. 182:459. (Block)
5. Liu Y, et al. 1997. J. Exp. Med. 185:251. (Block)
6. Herold KC, et al. 1997. J. Immunol. 158:984. (Block, IHC)
7. Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
8. Lawson BR, et al. 2007. J. Immunol. 178:5366.
9. Turnquist HR, et al. 2007. J. Immunol. 178:7018.
10. Klinger MB, et al. 2007. Am. J. Physiol. Requl. Integr. Comp. Physiol. 293:R677. PubMed
11. Verhagen J, et al. 2013. PNAS 110:E221. PubMed
12. Ma Y, et al. 2014. Cancer Res. 74:436. PubMed
13. Sharma SK, et al. 2015. J Immunol. 194:5529. PubMed

LPS-stimulated (3 days) C57BL/6 mouse

LPS-stimulated (3 days) C57BL/6 mouse splenocytes stained with GL-1 Alexa Fluor® 647



Description:

CD86 is an 80 kD immunoglobulin superfamily member also known as B7-2, B70, and Ly-58. CD86 is expressed on activated B and T cells, macrophages, dendritic cells, and astrocytes. CD86, along with CD80, is a ligand of CD28 and CD152 (CTLA-4). CD86 is expressed earlier in the immune response than CD80. CD86 has also been shown to be involved in immunoglobulin class-switching and triggering of NK cell-mediated cytotoxicity. CD86 binds to CD28 to transduce co-stimulatory signals for T cell activation, proliferation, and cytokine production. CD86 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells.

Other Names: B7-2, B70, Ly-58
Structure: Ig superfamily, 80 kD
Distribution: B cells and T cells (upregulated upon activation), macrophages, dendritic cells, and astrocytes
Function: T cell costimulation, Ig class-switching, NK cell cytotoxicity
Ligand Receptor: CD28, CD152 (CTLA-4)
Antigen References:

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Hathcock KS, et al. 1993. Science 262:905.
3. Freeman GJ, et al. 1993. Science 262:907.
4. Carreno BM, et al. 2002. Annu. Rev. Immunol. 20:29.


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