The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions.
Storage & Handling:
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested IHC , IF - Reported in the literature
The OX-7 antibody reacts with rat CD90 and mouse CD90.1 (Thy-1.1) (which is expressed by mouse strains of AKR/J, PL, and FVB/N), but not mouse CD90.2.
Additional reported applications (for the relevant formats) include: immunohistochemical7 and immunofluorescent8 staining of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, immunoprecipitation1, Western blotting1, in vitro activation of leukocytes2, induction of endothelial cell permeability3, induction of apoptosis in glomerular mesangial cells, and induction of glomerulonephritis in vivo4.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
1. Jeng CJ, et al. 1998. J. Cell Biol. 140:685. (IP, WB) 2. Nakashima I, et al. 1991. J. Immunol. 147:1153. 3. Ishizu A, et al. 1995. Int. Immunol. 7:1939. 4. Eitner F. 1997. Kidney. Int. 51:69. 5. Kawachi H, et al. 1992. Clin. Exp. Immunol. 88:399. (WB) 6. Dyer KD, et al. 2007. J. Immunol. 179:1693. (FC) PubMed 7. Daniel C, et al. 2012. Lab Invest. 92:812. (IHC) 8. Li B, et al. 2006. Kidney Int. 69:323. (IF)
LOU rat thymocytes stained with OX-7 Alexa Fluor® 647
CD90, also known as Thy-1, is a 28-30 kD GPI-linked membrane glycoprotein. It is a member of the immunoglobulin superfamily and has been shown to interact with CD45 in signal transduction during lymphocyte proliferation and differentiation. CD90 is expressed on hematopoietic stem cells, neurons, thymocytes, peripheral T cells, fibroblasts, stromal cells.
Hematopoietic stem cells, thymocytes, a subset of peripheral blood T cells, early myeloid and erythroid progenitors, immature B cells, neurons, activated endothelium, mast cells, dendritic cells, glomeruli
Adhesion, signal transduction, lymphocyte co-stimulation, proliferation and differentiation of hematopoietic stem cells
Interacts with CD45
1. Campbell DG, et al. 1981. Biochem. J. 195:15. 2. Hosseinzadeh H, et al. 1993. J. Immunol. 150:1670.
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