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Safety Data Sheet (SDS) Alexa Fluor 647 Anti-mouse CD106 Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 647 Anti-mouse CD106 Antibody
1128555 25 µg $90.00       
1128560 100 µg $190.00       
Clone: 429 (MVCAM.A)
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: Mouse preadipose cell line PA6
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested
IHC-F - Validated
IF - Reported in the literature

Application Notes:

Additional reported applications (for the relevant formats) include: immunohistochemical staining2,3,5-7 of acetone-fixed frozen sections, blocking4,5,8 of ligand binding in vitro and in vivo, and immunoprecipitation1.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. For immunohistochemical staining on frozen tissue sections, the suggested use is 5-10 µg/mL. It is recommended that the reagent be titrated for optimal performance for other applications.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.

Application References:

1. Kinashi T, et al. 1995. J. Leukoc. Biol. 57:168. (IP)
2. Koni PA, et al. 2001. J. Exp. Med. 193:741. (IHC)
3. Ishiyama N, et al. 1998. Pathobiology 66:274. (IHC)
4. Kinashi T, et al. 1994. Blood Cells 20:25. (Block)
5. Baron JL, et al. 1994. J. Clin. Invest. 93:1700. (Block IHC)
6. Buck CA, et al. 1996. Cell Adhes. Commun. 4:69. (IHC)
7. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
8. Meunier MC, et al. 2005. Nature Medicine 11:1222. (Block) PubMed
9. Nakagawa R, et al. 2013. J. Immunol. 190:3309. PubMed.

C57BL/6 bone marrow myeloid cells

C57BL/6 bone marrow myeloid cells stained with 429 Alexa Fluor® 647

C57BL/6 mouse frozen testis section

C57BL/6 mouse frozen testis section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of CD106 (clone 429 (MVCAM.A)) Alexa Fluor® 647 (green) and 10 µg/ml of CD117 (clone 2B8) Alexa Fluor® 594 (red) overnight at 4°C. Nuclei were counterstained with DAPI (blue). The image was captured by 10X objective.



Description:

CD106 is a 110 kD glycosylphosphatidylinositol (GPI)-linked transmembrane protein, also known as VCAM-1 and INCAM-110. It is constitutively expressed on bone marrow stromal cells, myeloid progenitors, splenic dendritic cells, activated endothelial cells, as well as some lymphocytes. CD106 expression can be upregulated on endothelial cells by inflammatory cytokines. CD106 is involved in adhesion and acts as a counter-receptor for VLA-4 (α41 integrin) and LPAM-1 (α47 integrin). The 429 antibody has been reported to partially block VCAM-1-mediated binding.

Other Names: VCAM-1, INCAM-110
Structure: Ig superfamily, 47 kD
Distribution: Bone marrow stromal cells, myeloid progenitors, splenic dendritic cells, activated endothelial cells
Function: Adhesion
Ligand Receptor: VLA-4 (α41 integrin) and LPAM-1 (α47 integrin)
Antigen References:

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Kinashi T, et al. 1995. J. Leukoc. Biol. 57:168.
3. Bevilacquea MP. 1993. Annu. Rev. Immunol. 11:767.
4. Koni PA, et al. 2001. J. Exp. Med. 193:741.


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